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SAB4200746

Sigma-Aldrich

Anti-α-Fetoprotein (AFP) antibody, Mouse monoclonal

clone C3, purified from hybridoma cell culture

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Synonym(s):
Anti-AFP, Anti-Alpha-1-fetoprotein, Anti-Alpha-fetoglobulin
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

unconjugated

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

C3, monoclonal

form

buffered aqueous solution

mol wt

~74 kDa

species reactivity

human, canine, porcine

concentration

~1.0 mg/mL

technique(s)

ELISA: suitable
dot blot: suitable
immunoblotting: 2-4 μg/mL using liver hepatocellular carcinoma HepG2 cell line extract
immunofluorescence: suitable
immunohistochemistry: 2-4 μg/mL using formalin-fixed, paraffin-embedded human fetal liver sections

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... AFP(174)

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This Item
SAB3500533WH0000174M1SAB4700975
clone

C3, monoclonal

clone

polyclonal

clone

1G7, monoclonal

clone

AFP-01, monoclonal

Gene Information

human ... AFP(174)

Gene Information

human ... AFP(174)

Gene Information

human ... AFP(174)

Gene Information

human ... AFP(174)

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

biological source

mouse

biological source

rabbit

biological source

mouse

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

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General description

The gene encoding α-fetoprotein (AFP) is localized on human chromosome 4. It encodes a plasma glycoprotein that has a molecular mass of 70kDa. It is synthesized along with serum albumin during the development of embryonic yolk sac and the liver.

Immunogen

Purified human α-fetoprotein

Biochem/physiol Actions

α-fetoprotein (AFP) is involved in the regulation of fatty acids in both fetal and proliferating adult liver cells. Its expression is found to be increased in acute liver injuries, indicating active liver regeneration. AFP has been associated with fatty liver disease (FLD), a disease that may lead to cirrhosis and hepatocellular carcinoma. It serves as a tumor marker for HCC (hepatocellular carcinoma).

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

Store at –20°C. For continuous use, store at 2–8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

In order to obtain best results in different techniques and preparations, we recommend determining optimal working concentration by titration test.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Evaluation of alpha-fetoprotein in detecting hepatocellular carcinoma recurrence after radiofrequency ablation.
Siripongsakun S, et al.
Journal of Gastroenterology and Hepatology (2014)
Linkage of the evolutionarily-related serum albumin and alpha-fetoprotein genes within q11-22 of human chromosome 4.
M E Harper, et al.
American Journal of Human Genetics (1983)
Clinical significance of elevated serum alpha-fetoprotein (AFP) level in acute viral hepatitis A (AHA).
Seo SI, et al.
Hepato-Gastroenterology (2013)
Ana Gil-Bona et al.
International journal of molecular sciences, 24(21) (2023-11-14)
Excess albumin in enamel is a characteristic of the prevalent developmental dental defect known as chalky teeth or molar hypomineralization (MH). This study uses proteomic analyses of pig teeth to discern between developmental origin and post-eruptive contamination and to assess
Association between serum alpha-fetoprotein levels and fatty liver disease: a cross-sectional study.
Xu P, et al.
World Journal of Gastrointestinal Oncology (2014)

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