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SAB4200748

Sigma-Aldrich

Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse

clone MB-11, purified immunoglobulin

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Synonym(s):
Anti-Human immunoglobulin M
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified immunoglobulin

antibody product type

primary antibodies

clone

MB-11, monoclonal

form

lyophilized powder

species reactivity

human

concentration

~2.0 mg/mL

technique(s)

ELISA: 1:40,000-1:80,000 using 2.5 μg/mL Human IgM for coating

isotype

IgG2b

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
SAB4200755SAB4200791SAB4200743
clone

MB-11, monoclonal

clone

GE-1, monoclonal

clone

GG-6, monoclonal

clone

HP-6017, monoclonal

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

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General description

Monoclonal Anti-Human IgM (mouse IgG2b isotype) is derived from the MB-11 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a BALB/c mouse immunized with purified human IgM protein. Immunoglobulin M (IgM) is the major class of surface immunoglobulins on lymphocytes membranes. IgM has a pentameric structure, in which monomers are linked together via disulfide bonds. Surface IgM is expressed on immature and mature B cells, whereas IgM heavy chain is expressed intracellularly in pre-B cells. IgM serves as the antigen receptor of naive B cells.

Specificity

Monoclonal Anti-Human IgM (μ-chain) specifically recognizes μ chain of Human IgM. The antibody shows no cross-reactivity with human IgG, Fab, Fc, kappa, lambda and gamma chains.

Immunogen

purified human IgM protein

Application

Monoclonal Anti-Human IgM (μ-chain specific)-Peroxidase antibody produced in mouse may suitable for use in enzyme-linked immunosorbent assay (ELISA).

Biochem/physiol Actions

IgM is suggested to act as the first line of defense during microbial infections. Secreted IgM (sIgM) is involved in B cell maturation and complement activation. Detection of IgM antibodies in a patient′s serum may indicates a recent infection and IgM in a neonate′s serum may indicate on intrauterine infection (e.g. congenital rubella syndrome). In addition, the development of anti-donor IgM after combined liver-kidney transplantation has been shown to provide a graft- protecting effect. Anti-Human IgM (μ-specific) antibodies can be used in detection of the abnormal concentration of IgM in patient sera and IgM-associated pathologies, such as selective IgM immunodeficiency (SIgMD) and the hyperimmunoglobulin M (hyper-IgM or HIGM) syndromes.

Physical form

Supplied as a lyophilized powder

Storage and Stability

Store the lyophilized product at 2–8 °C. For extended storage after reconstitution, keep at –20 °C in working aliquots. Avoid repeated freeze-thaw cycles. For continuous use after reconstitution, keep at 2–8 °C for up to 1 month. Solutions at working dilution should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Natural antibodies: from first-line defense against pathogens to perpetual immune homeostasis
Maddur MS, et al.
Clinical Reviews in Allergy & Immunology, 58(2), 213-228 (2020)
Protective anti-donor IgM production after crossmatch positive liver-kidney transplantation
McAlister CC, et al.
Liver Transplantation, 10(2), 315-319 (2004)
Ana Cristina Rodrigues et al.
Bioresource technology, 156, 163-169 (2014-02-08)
The potential of enzymes recycling after hydrolysis and fermentation of wheat straw under a variety of conditions was investigated, monitoring the activity of the enzymes in the solid and liquid fractions, using low molecular weight substrates. A significant amount of
Dorrah Deeb et al.
International journal of oncology, 49(3), 1139-1147 (2016-08-31)
Pancreatic ductal adenocarcinoma (PDA) remains one of the most difficult to treat of all malignancies. Multimodality regimens provide only short-term symptomatic improvement with minor impact on survival, underscoring the urgent need for novel therapeutics and treatment strategies for PDA. Trichothecenes
Maria C Dzialo et al.
The Journal of biological chemistry, 289(44), 30511-30524 (2014-09-19)
Methylation of various components of the translational machinery has been shown to globally affect protein synthesis. Little is currently known about the role of lysine methylation on elongation factors. Here we show that in Saccharomyces cerevisiae, the product of the

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