SAB4200754

Sigma-Aldrich

Anti-IL6 antibody produced in rabbit

IgG fraction of antiserum, buffered aqueous solution

Synonym(s):
Anti-CTL differentiation factor (CDF), Anti-Hybridoma growth factor, Anti-B-cell stimulatory factor 2 (BSF-2), Anti-Interferon beta-2 (IFN-beta-2), Anti-Interleukin-6
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

IgG fraction of antiserum

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~23 kDa

species reactivity

human

concentration

~1.0 mg/mL

application(s)

immunoblotting: 1:2,000-1:4,000 using human osteosarcoma MG-63 concentrated supernatant treated with IL1ß
immunoprecipitation (IP): 1-2.5 μL using human osteosarcoma MG-63 concentrated supernatant treated with IL1ß

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

Gene Information

human ... IL6(3569)

General description

Interleukin-6 (IL6) is a multifunctional cytokine. The gene is located on the human chromosome at 7p15.3. IL6 is found in many supplemental cloning media.

Specificity

Anti-IL6 specifically recognizes human IL6.

Immunogen

recombinant IL6

Application

Anti-IL6 antibody produced in rabbit may be used in immunoblotting and immunoprecipitation.

Biochem/physiol Actions

Interleukin-6 (IL6) plays a wide variety of biological roles in the immune , nervous and endocrine systems. It is involved as a primary regulator of acute and chronic inflammatory diseases. IL6 is a target for clinical treatment against autoimmune disorders such as rheumatoid arthritis, juvenile idiopathic arthritis and Castleman′s disease. It also plays a key role in the pathogenesis of myeloma. IL6 levels are higher in patients with different kinds of malignant disease. Elevated circulating levels of IL6 is considered as a biomarker for acute coronary syndrome (ACS), cardiovascular diseases, such as heart attack and stroke and unstable angina. It is an essential component of hybridoma growth.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
The role of immunotherapy in multiple myeloma
Kocoglu M and Badros A
Pharmaceuticals (Basel, Switzerland), 9(1), 3-3 (2016)
Interleukin-6 and chronic inflammation
Gabay C
Arthritis Research & Therapy, 8(S2), S3-S3 (2006)
Elevated levels of interleukin-6 in unstable angina
Biasucci L M, et al.
Circulation, 94(5), 874-877 (1996)
Systemic inflammation in C57BL/6J mice receiving dietary aluminum sulfate; up-regulation of the pro-inflammatory cytokines IL-6 and TNF alpha, C-reactive protein (CRP) and miRNA-146a in blood serum
Pogue A I, et al.
Journal of Alzheimer's Disease & Parkinsonism, 7(6), 874-877 (2017)
Inflammatory biomarkers in acute coronary syndromes: part I: introduction and cytokines.
Ehrin J Armstrong et al.
Circulation, 113(6), e72-e75 (2006-02-16)

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