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Anti-AsCas12a (Cpf1) antibody, Mouse monoclonal

clone AsCpf-11, purified from hybridoma cell culture

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Anti-AsCpf1 antibody, Mouse monoclonal, Anti-CRISPR-associated endonuclease AsCas12a from Acidaminococcus sp. (strain BV3L6)

biological source


Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies


AsCpf-11, monoclonal


buffered aqueous solution

mol wt

~135 kDa


~1.0 mg/mL


immunoblotting: 1.25-2.5 μg/mL using purified recombinant AsCpf1 produced in E. coli
immunofluorescence: 1.25-2.5 μg/mL using human HEK-293T cells over-expressing AsCpf1 protein
immunoprecipitation (IP): 2.5-5 μg/test using lysate of human HEK-293T cells over-expressing AsCpf1 protein



UniProt accession no.

shipped in

dry ice

storage temp.


target post-translational modification


General description

Clustered, regularly interspaced, short palindromic repeat (CRISPR) systems, encode RNA-guided endonucleases that are essential for bacterial adaptive immunity. Ddepending on the architecture of the effector-CRISPR RNA (crRNA) interference module, the ddifferent CRISPR-Cas systems could be assigned into two classes: Class-1 systems of multi-subunit complex such as Cascade and Class-2 systems of single enzyme, such as Cas9.
Cpf1 (CRISPR from Prevotella and Francisella 1) belongs to Class-2 type V CRISPR-Cas endonuclease system.4-5 Cpf1 comprise several differences from Cas9 protein including cleavage with 5′overhangs, a shorter guide RNA and a longer distance between the seed sequence and the cleavage site.
AsCpf1, Cpf1 from Acidaminococcus sp. (strain BV3L6), was examined together with 15 members of Cpf1 nuclease family and shown to mediate efficient genome editing in HEK293FT cells with an improved results compared to SpCas9.5 AsCpf1 crystal structure in a complex with crRNA and partially duplexed target DNA, shows that AsCpf1 acts as a monomer thus identifying a unique mechanism employed by AsCpf1 for target recognition.


recombinant Cpf1 from Acidaminococcus sp. (strain BV3L6)


Monoclonal Anti-AsCpf1 recognizes Cpf1 from Acidaminococcus sp. (strain BV3L6). The product has been used in several immunochemical techniques including
  • immunoblotting (~135 kDa),
  • immunofluorescence
  • immunoprecipitation

Monoclonal anti-AsCpf1 antibody can provide a useful tool for genome editing research such as detecting and monitoring AsCpf1 positively transfected cells.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.
In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

Storage Class

10 - Combustible liquids


Not applicable


Not applicable

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Engineering cell signaling using tunable CRISPR-Cpf1-based transcription factors
Liu Y, et al.
Nature Communications, 8(1), 2095-2095 (2017)
Yuchen Liu et al.
Nature communications, 8(1), 2095-2095 (2017-12-14)
The catalytically dead Cpf1 endonuclease from Acidaminococcus sp. BV3L6 (dAsCpf1) has been used to construct effective transcriptional repressors in bacteria and plants. However, it is still unclear if dAsCpf1 can function in human cells as a transcriptional regulator or a

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