Anti-Vimentin antibody, Mouse monoclonal

clone LN-6, purified from hybridoma cell culture


Quality Level

biological source


antibody product type

primary antibodies


LN-6, monoclonal


buffered aqueous solution

mol wt

~58 kDa

species reactivity

rabbit, mouse, hamster, rat, porcine, bovine, monkey, human, canine


~1 mg/mL


flow cytometry: suitable
immunoblotting: 0.25-0.5 μg/mL using human foreskin fibroblast Hs68 cell line.
immunofluorescence: 1-2 μg/mL using human foreskin fibroblast Hs68 cell line.
immunohistochemistry: 5-10 μg/mL using Human breast carcinoma sections.
immunoprecipitation (IP): suitable



UniProt accession no.

shipped in

dry ice

storage temp.


General description

Anti-Vimentin antibody, Mouse monoclonal (mouse IgM isotype) is derived from the LN-6 hybridoma produced by the fusion of mouse myeloma cells and splenocytes from mouse. Vimentin protein is localized in a wide range of cell types, including pancreatic precursor cells, sertoli cells, mesangial cells, trophoblastic giant cells, neuronal precursor cells, fibroblasts, macrophages, endothelial cells of lung blood vessels, renal tubular cells, neutrophils, leukocytes and renal stromal cells.
Vimentin is a specific type III intermediate filament (IF) protein. It is abundantly expressed in mesenchymal cells. The gene encoding vimentin is located on human chromosome 10p13.


Anti-Vimentin antibody, Mouse monoclonal specifically recognizes a unique epitope of Vimentin protein which is not expressed in cells of hematopoetic derivation. The antibody is reactive with benign and malignant tumors of mesenchymal and neural derivation but does not react with normal and malignanat human lymphoid cells or derived malignancies. The antibody reacts with Vimentin from human, hamster, mouse, rat, canine, monkey, bovine, porcine and rabbit origin.


Human thymic nuclear extract


Anti-Vimentin antibody, Mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • flow cytometry
  • immunoprecipitation
  • immunohistochemistry

Biochem/physiol Actions

Vimentin protein plays a significant role in supporting and anchoring the cellular position of organelles such as the nucleus, endoplasmic reticulum, mitochondria, maintenance of cell shape, the endurance of mechanical stress of mesenchymal cells and motility. Overexpression of the vimentin gene is observed in various epithelial cancers, including prostate, gastrointestinal tumors, central nervous system (CNS) tumors, breast, malignant melanoma and lung cancer.
Vimentin helps to preserve the structure of cells and stability of tissue in mesenchymal cells. It is involved in tumorigenesis, EMT (epithelial mesenchymal transition) and the metastatic of cancer. It also controls infectious internalization of human papillomavirus 16 pseudovirions.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.


Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.


NONH for all modes of transport

WGK Germany


Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
Daying Dai et al.
AJNR. American journal of neuroradiology, 26(10), 2560-2568 (2005-11-16)
The purpose of this study was to clarify the cellular mechanisms of aneurysmal healing by comparing histologic and immunohistochemical findings in experimental rabbit and swine aneurysms to a human aneurysm embolized with platinum coils. Swine sidewall aneurysms (n = 5...
Jui M Dave et al.
Microcirculation (New York, N.Y. : 1994), 21(4), 333-344 (2014-01-07)
Angiogenesis is a multistep process that requires intricate changes in cell shape to generate new blood vessels. IF are a large family of proteins that play an important structural and functional role in forming and regulating the cytoskeleton. Vimentin, a...
The gene encoding human vimentin is located on the short arm of chromosome 10.
Ferrari S E R G I O, et al.
American Journal of Human Genetics, 41(4), 616-616 (1987)
Vimentin modulates infectious internalisation of HPV16 pseudovirions.
Schafer G, et al.
Journal of Virology, 91(16), e00307-e00317 (2017)
Audrey Gilson et al.
Arthritis research & therapy, 12(1), R24-R24 (2010-02-16)
Development of cell therapies for repairing the intervertebral disc is limited by the lack of a source of healthy human disc cells. Stem cells, particularly mesenchymal stem cells, are seen as a potential source but differentiation strategies are limited by...

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