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SAB4200767

Sigma-Aldrich

Anti-Cathepsin D antibody, Mouse monoclonal

clone CTD-19, purified from hybridoma cell culture

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Synonym(s):
Anti-CTSD, cleaved into the following 2 chains: Cathepsin D light chain and Cathepsin D heavy chain
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

CTD-19, monoclonal

form

buffered aqueous solution

species reactivity

human, rabbit

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2-4 μg/mL using human breast cancer MCF7 cell line
immunofluorescence: 5-10 μg/mL using HeLa cells
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human liver sections

isotype

IgG2a

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... CTSD(1509)

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This Item
C0715C4618SAB4200731
antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

purified from hybridoma cell culture

clone

CTD-19, monoclonal

clone

CTD-19, monoclonal

clone

CPL33/1, monoclonal

clone

CH-19, monoclonal

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

species reactivity

human, rabbit

species reactivity

human

species reactivity

human

species reactivity

guinea pig, canine, frog, bovine, human, cat, monkey, rabbit, mouse, ferret, snake, hamster, chicken, Psammomys (sand rat), rat

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

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General description

Anti-Cathepsin D antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the CTD-19 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from BALB/c mouse immunized with cathepsin D purified from human liver. Cathepsin D (CatD or CTSD) is synthesized in the rough endoplasmic reticulum as pre-proprotein. After removal of signal peptide, the pro-CatD is targeted to endosomes to form an active, ~48 kDa, single-chain intermediate then to the lysosomes to form the fully active mature protease, composed of a ~30 kDa heavy chain and a ~14 kDa light chain.

Immunogen

Cathepsin D Purified from human liver

Application

Anti-Cathepsin D antibody, Mouse monoclonal has been used in:
  • immunoblotting
  • immunohistochemistry
  • immunofluorescence

Biochem/physiol Actions

CatD plays numerous physiological functions in the cells including metabolic degradation of intracellular proteins and the activation of enzymatic precursors. In the central nervous system, CatD is particularly important for the control of neuronal homeostasis, cell migration and interneuron communication. CatD-mediated proteolysis mediates the degradation of unfolded/oxidized protein aggregates in lysosome. The level of CatD synthesized by the cells is increased in response to mitogenic signals from estrogen, epidermal growth factor (EGF), fibroblast growth factor (FGF), and insulin like growth factor-I (IGF- I). The ability of tumor cells to invade the extracellular matrix has been attributed to cathepsins released by tumor cells or associated with its plasma membrane.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage Class

10 - Combustible liquids

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cathepsin D as a therapeutic target in Alzheimer's disease.
Di Domenico F, et al.
Expert Opinion on Therapeutic Targets, 20(12), 1393-1395 (2016)
Veronika Stoka et al.
Ageing research reviews, 32, 22-37 (2016-04-30)
Lysosomes and lysosomal hydrolases, including the cathepsins, have been shown to change their properties with aging brain a long time ago, although their function was not really understood. The first biochemical and clinical studies were followed by a major expansion
The role of cathepsin D in the pathogenesis of human neurodegenerative disorders
Vidoni C, et al.
Medicinal Research Reviews, 36(5), 845-870 (2016)
Nuclear cathepsin D enhances TRPS1 transcriptional repressor function to regulate cell cycle progression and transformation in human breast cancer cells
Bach AS, et al.
Oncotarget, 6(29), 28084-28084 (2015)
Clivia Lisowski et al.
Autophagy, 18(8), 1785-1800 (2021-11-17)
Modulation of the host cell cycle has emerged as a common theme among the pathways regulated by bacterial pathogens, arguably to promote host cell colonization. However, in most cases the exact benefit ensuing from such interference to the infection process

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