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SAB4200768

Sigma-Aldrich

Anti-Human IgG1-Peroxidase antibody, Mouse monoclonal

clone 8c/6-39, purified from hybridoma cell culture

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Synonym(s):
Anti-Human immunoglobulin G1
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

8c/6-39, monoclonal

form

lyophilized

species reactivity

human

concentration

~2 mg/mL

technique(s)

ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating

isotype

IgG2a

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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1 of 4

This Item
SAB4200769SAB4200790SAB4200770
clone

8c/6-39, monoclonal

clone

HP-6050, monoclonal

clone

GG-7, monoclonal

clone

HP-6025, monoclonal

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

technique(s)

ELISA: 1:8,000-1:16,000 using 5 μg/ml human IgG1 for coating

technique(s)

direct ELISA: 1:80,000-1:160,000 using 2 μg/mL human IgG3 for coating.

technique(s)

direct ELISA: 1:40,000-1:80,000 using 2.5 μg/mL human IgG1 for coating

technique(s)

ELISA: 1:80,000-1:160,000 using 1 μg/mL human IgG4 for coating

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General description

Human IgGs consist of four subclasses (1-4) that can be recognized by antigenic differences in their heavy chains. They constitute approximately 65, 30, 5, and 4% of the total IgG, respectively. Each subclass has different biological and physiochemical properties.Monoclonal Anti-Human IgG1 specifically recognizes Human IgG1. The antibody shows no cross-reactivity with human IgG2, IgG3, or IgG4. This clone has been established as a useful human IgG1 specificity standard by the WHO/IUIS.
Monoclonal Anti-Human IgG1 (mouse IgG2a isotype) is derived from the 8c/6-39 hybridoma (also known as HP6019), produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with the Fc fragment of a human IgG1 myeloma protein.

Immunogen

The Fc fragment of a human IgG1 myeloma protein

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Biochem/physiol Actions

The immunoglobin G (IgG) subclass may be preferentially produced in response to different antigens and pathological conditions. For instance, anti-polysaccharide responses are mainly of the IgG2 subclass while protein antigens give rise to IgG1 and IgG3 antibodies. Human IgG1 is the main antibody produced against tetanus toxoid antibodies. Elevation of IgG1 levels has been found in the cerebral spinal fluid of patients with multiple sclerosis.

Physical form

Supplied as lyophilized powder. After reconstitution with 0.1 mL of distilled water to a final antibody concentration of ∼ 2 mg/mL, the solution contains 1% BSA, 2.5% trehalose, 0.05% MIT in 0.01 M sodium phosphate buffered saline

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

13 - Non Combustible Solids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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Cerebrospinal fluid total tau protein levels in patients with multiple sclerosis
Terzi M, et al.
Acta Neurologica Scandinavica, 115(5), 325-330 (2007)
Structure and function of immunoglobulins.
Schroeder Jr H W and Cavacini L
The Journal of Allergy and Clinical Immunology, 125(2), S41-S52 (2010)

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