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SAB4200776

Sigma-Aldrich

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal

clone TUB-1A2, purified from hybridoma cell culture

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Synonym(s):
Tubulin Alpha-1 Chain
NACRES:
NA.41

biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

TUB-1A2, monoclonal

form

buffered aqueous solution

mol wt

~55 kDa

species reactivity

Xenopus, Arabidopsis sp., yeast, insect, rat, hamster, porcine, monkey, bovine, human, mouse, canine, chicken

concentration

~1 mg/mL

technique(s)

immunoblotting: suitable
immunofluorescence: 2-4 μg/mL using human HeLa or Chicken fibroblast cells
immunohistochemistry: suitable

isotype

IgG3

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... TUBA1A(7846)

General description

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal (mouse IgG3 isotype) is derived from the TUB-1A2 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse. Tubulin is an intracellular cylindrical filamentous structure and is presented in eukaryotic cells. It consists of three isoforms α-, β- and γ-tubulin. Tubulin is a heterodimer of α- and β- tubulin subunits.

Specificity

Anti-Tubulin, Tyrosine antibody, Mouse monoclonal specifically recognizes the C-terminal Tyrosine of Tubulin protein. The antibody is non-reactive with cells that have been treated with pancreatic carboxypeptidase A under conditions which remove only the C-terminal tyrosine. Monoclonal Anti-Tubulin, Tyrosine recognizes Tubulin from human, mouse, chicken, bovine, rat, hamster, canine, monkey, porcine, arabidopsis, tobacco, yeast, Xenopus, insect, comb jelly, sponge, and worm origin.

Immunogen

a synthetic peptide containing the carboxy terminal amino acids of porcine a-tubulin

Application

Anti-Tubulin, Tyrosine antibody, mouse monoclonal may be used in:
  • immunoblotting
  • immunofluorescence
  • immunohistochemistry

Biochem/physiol Actions

Tubulin is the major building block of microtubules which is essential for structural and mobility elements in mitosis, intracellular transport, flagellar movement and in the cytoskeleton. It is also involved in the nervous system. Tubulin is associated with neurodegenerative disorders such as Alzheimer′s and Parkinson′s disease. Mutations in the αβ- dimers and γ-tubulin are associated with malformation and impairment of cognitive disorders.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide.

Storage and Stability

For continuous use, store at 2–8 °C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog, our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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Tubulin: Structure, Functions and Roles in Disease
Binarova P and Tuszynski J
Cells, 10(3), 194-204 (2019)
Microtubules and resistance to tubulin-binding agents
Kavallaris M
Nature Reviews. Cancer, 10(3), 194-204 (2010)

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