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SAB4200778

Sigma-Aldrich

Anti-NRAS (internal) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Anti-GTPase NRas, Anti-transforming protein N-Ras
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

predicted mol wt ~21 kDa

species reactivity

human

concentration

~1.0 mg/mL

technique(s)

immunoblotting: 2.5-5 μg/mL using human MCF7 cell line extract
immunohistochemistry: 10-20 μg/mL using heat-retrieved formalin-fixed, paraffin-embedded human melanoma sections

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... NRAS(4893)

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This Item
WH0004893M1SAB5500153SAB4100599
antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

affinity isolated antibody

antibody form

purified from hybridoma cell culture

clone

polyclonal

clone

2A3, monoclonal

clone

SP174, monoclonal

clone

KRAS-A582, monoclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

-

form

aqueous solution

mol wt

predicted mol wt ~21 kDa

mol wt

-

mol wt

-

mol wt

antigen predicted mol wt 27.9 kDa

species reactivity

human

species reactivity

human

species reactivity

human (tested)

species reactivity

human

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General description

NRAS, also known as GTPase NRas, Transforming protein N-Ras, or Neuroblastoma RAS Viral (V-Ras) Oncogene Homolog, is a member of the Ras protein family, together with KRAS (Kirsten RAS), and HRAS (Harvey RAS), comprising a family of low-molecular-weight GTPases. The Ras family is named for a retrovirus that induced rat sarcomas that were later found to have activating RAS mutations. Ras family proteins serve as molecular switches in regulating pathways that are responsible for diverse cellular processes such as proliferation, differentiation, migration, and apoptosis.3 Ras proteins are highly homologous regarding their primary amino acid sequence and the differences among them concentrated in their C-terminal region. Anti-NRAS (internal) antibody produced in rabbit specifically recognizes human NRAS and does not cross-react with KRAS.

Immunogen

Synthetic peptide from the internal region of human NRAS protein, conjugated to KLH

Application

The antibody may be used in various immunochemical techniques including immunoblotting (predicted ∼21 kDa) and immunohistochemistry. Detection of the NRAS band by Immunoblotting is specifically inhibited by the immunizing protein.

Physical form

Solution in 0.01 M phosphate buffered saline, pH 7.4, containing 15 mM sodium azide as preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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