SAB4200789

Sigma-Aldrich

Anti-Human Lambda Light Chains (Bound and Free)-Peroxidase antibody, Mouse monoclonal

clone HP-6054, purified from hybridoma cell culture

Synonym(s):
Anti-Human immunoglobulin lambda light chain
NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

mouse

antibody product type

primary antibodies

clone

HP-6054, monoclonal

form

lyophilized powder

species reactivity

human

concentration

~2 mg/mL

application(s)

direct ELISA: 1:16,000-1:32,000 using using 2 μg/ml human IgG lambda light chains for coating.

isotype

IgG2a

conjugate

peroxidase conjugate

shipped in

dry ice

storage temp.

−20°C

General description

Anti-Human Lambda Light Chains (Bound and Free)- Peroxidase antibody, Mouse monoclonal (mouse IgG2a isotype) is derived from the HP-6054 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from a mouse immunized with purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads. Immunoglobulins are composed of two heavy and two light polypeptide chains held together by noncovalent forces and usually by interchain disulfide bridges. The various types of human (and other mammalian) immunoglobulins contain one of the two existing light chain types, either κ or λ in which multiple structural differences are reflected in antigenic variety, mainly the N-terminal (variable) domain of the chains.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2 μg/mL human IgG lambda light chains for coating.

Biochem/physiol Actions

In monoclonal disorders, such as myeloma and macroglobulinemia, an increase in the level of a single immunoglobulin class can be accompanied by disproportionate increase in either λ or κ light chains. In many cases of B cell malignancy there is an increased production of light chains which are not accompanied with heavy chains. These circulate in the various body fluids (blood, cerebral spinal fluid, and tissues) and are also found in enormous amounts as free molecules in the urine (Bence Jones paraproteins).

Physical form

Supplied as a lyophilized powder.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin
The laboratory investigation of paraproteinaemia.
J T Whicher et al.
Annals of clinical biochemistry, 24 ( Pt 2), 119-132 (1987-03-01)
C M Black et al.
Journal of immunological methods, 106(1), 71-81 (1988-01-21)
Improvements were made in the range, precision, convenience, automation, and reliability of our previously published two-site immunoenzymometric assays using mouse monoclonal antibodies specific for human IgG and its subclasses. The serum concentration ratios for these immunoglobulin isotypes were measured in...
D J Phillips et al.
Immunology letters, 17(2), 159-168 (1988-02-01)
We measured the association constant (Ka) for 42 murine monoclonal antibodies (MAbs) to human IgG epitopes. Included are antibodies, previously evaluated in an IUIS/WHO collaborative study, to various epitopes on the four subclasses of human IgG - IgG Fc, IgG...
C B Reimer et al.
Hybridoma, 3(3), 263-275 (1984-01-01)
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2...

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