SAB4200790

Sigma-Aldrich

Anti-Human IgG (Fc specific)-Peroxidase antibody, Mouse monoclonal

clone GG-7, purified from hybridoma cell culture

Synonym(s):
Anti-Human immunoglobulin G
NACRES:
NA.46

Quality Level

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GG-7, monoclonal

form

lyophilized powder

species reactivity

human

application(s)

direct ELISA: 1:40,000-1:80,000 using 2.5 μg/mL human IgG1 for coating

isotype

IgG1

conjugate

peroxidase conjugate

shipped in

dry ice

storage temp.

−20°C

General description

IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid, and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent and every subclass has a specific biological function. IgG deficiencies are often associated with various diseases.

The Fc fragment has various important functions such as complement fixation, site for rheumatoid factor (autoantibody directed to Fc) attachment, passage through the placental membrane and protein A binding. A certain population of lymphocytes also possess an "Fc receptor". These functions indicate the importance of immunoreagents specific for the Fc fragment of Human IgG.

Monoclonal Anti-Human IgG (Fc specific) is specific for the Fc fragment of Human IgG and is observed with all human IgG subclasses but not with the Fab fragment of human IgG, purified light chains, human IgA or human IgM.

Immunogen

Purified human IgG myeloma proteins covalently coupled to polyaminostyrene (PAS) microbeads.

Application

The antibody is recommended to use in various immunological techniques, including ELISA.

Physical form

Supplied as a lyophilized powder

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

R Jefferis et al.
Annales de biologie clinique, 52(1), 57-65 (1994-01-01)
Secondary systemic immune responses are predominantly of the IgG class and passive administration of intravenous IgG, from pooled normal serum, is an effective prophylactic and/or therapeutic treatment for patients with defined immunodeficiencies. However, the proportions of each IgG subclass present...
C B Reimer et al.
Hybridoma, 3(3), 263-275 (1984-01-01)
Stable clones of 31 mouse hybridomas that produce monoclonal antibodies (MAbs) against human IgG antigenic determinants were obtained. The number of hybridomas of different specificity described are: 2 anti-IgG1 Fc, 1 anti-IgG2 Fc, 1 anti-IgG2 Fd, 2 anti-IgG3 Fc, 2...
C Papadea et al.
Critical reviews in clinical laboratory sciences, 27(1), 27-58 (1989-01-01)
Human IgG consists of two identical heavy (H) chains and two identical light (L) chains joined by interchain disulfide bridges. Heterogeneity in the amino acid sequences of the H and L polypeptides results in at least three types of IgG...

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