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SAB4200791

Sigma-Aldrich

Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal

clone GG-6, purified from hybridoma cell culture

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Synonym(s):
Anti-Human immunoglobulin G
NACRES:
NA.46

biological source

mouse

Quality Level

conjugate

peroxidase conjugate

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

GG-6, monoclonal

form

lyophilized powder

species reactivity

human

concentration

~2 mg/mL

technique(s)

direct ELISA: 1:16,000-1:32,000 using 2.5 μg/ml human IgG for coating.

isotype

IgG1

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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SAB4200743SAB4200790SAB4200755
biological source

mouse

biological source

mouse

biological source

mouse

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

antibody form

purified from hybridoma cell culture

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

conjugate

peroxidase conjugate

species reactivity

human

species reactivity

human

species reactivity

human

species reactivity

human

concentration

~2 mg/mL

concentration

~2 mg/mL

concentration

-

concentration

-

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General description

Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG (Fab fragment). IgGs are the most common Immunoglobulins isotype in blood, lymph fluid, cerebrospinal fluid and peritoneal fluid and a key players in the humoral immune response. IgGs include four subclasses (IgG1, IgG2, IgG3, and IgG4), they consist of a variable Fab fragment (which includes the antibody recognition site), and a conserved Fc fragment. The IgG subclasses differ in their physical and chemical properties, their distribution pattern is found to be age-dependent, and every subclass has a specific biological function.
Anti-Human IgG (Fab specific)-Peroxidase antibody, Mouse monoclonal (mouse IgG1 isotype) is derived from the GG-6 hybridoma, produced by the fusion of mouse myeloma cells and splenocytes from mouse immunized with purified human IgG.

Immunogen

Purified human IgG (Fab Fragment)

Application

Direct ELISA: a working dilution of 1:16,000-1:32,000 is recommended using 2.5 μg/mL human IgG for coating.

Biochem/physiol Actions

Immunoglobulin G (IgG) participates in hypersensitivity type II and type III reactions. Maternal IgG is the only antibody transported across the placenta to the fetus. Maternal IgG passively immunizes the infants. IgG deficiencies are often associated with various diseases.

Physical form

Supplied as a lyophilized powder.

Other Notes

In order to obtain best results in different techniques and preparations we recommend determining optimal working concentration by titration test.

pictograms

Exclamation mark

signalword

Warning

hcodes

Hazard Classifications

Skin Sens. 1

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


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