MilliporeSigma
All Photos(2)

Documents

SAB4200808

Sigma-Aldrich

Anti-Myosin Light Chain Kinase antibody, Mouse monoclonal

clone K36, purified from hybridoma cell culture

Sign Into View Organizational & Contract Pricing

Synonym(s):
Kinase-related protein (KRP), MLCK, Myosin light chain kinase, Smooth muscle, Telokin, smMLCK
NACRES:
NA.41

biological source

mouse

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

K36, monoclonal

form

buffered aqueous solution

mol wt

~160 kDa

species reactivity

bovine, human, mouse, chicken, pig, turkey

packaging

antibody small pack of 25 μL

concentration

~1 mg/mL

technique(s)

immunoblotting: 0.25-0.5 μg/mL using chicken gizzard extract

isotype

IgG2b

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
M7905WH0004638M1SAB1300116
clone

K36, monoclonal

clone

K36, monoclonal

clone

1D1, monoclonal

clone

polyclonal

antibody form

purified from hybridoma cell culture

antibody form

ascites fluid

antibody form

purified immunoglobulin

antibody form

IgG fraction of antiserum

biological source

mouse

biological source

mouse

biological source

mouse

biological source

rabbit

UniProt accession no.

Q15746

UniProt accession no.

-

UniProt accession no.

Q15746

UniProt accession no.

-

form

buffered aqueous solution

form

-

form

buffered aqueous solution

form

buffered aqueous solution

Still not finding the right product?  

Give our Product Selector Tool a try.

General description

Myosin Light Chain Kinase (MLCK) also known as MYLK is a Ca2+/calmodulin dependent myosin light chain phosphorylating agent. This enzyme plays a major role in the phosphorylation of the regulatory light chains of myosin which are essential for the shortening and tension development of smooth muscle cells resulting in smooth muscle contraction. Myosin light chain kinase are widely expressed in many different tissues and cells of eukaryote species. There are two genes mylk1 and mylk2 encoding the MLCK protein, mylk2 is exclusively expressed in skeletal muscle cells.

Immunogen

Purified chicken gizzard myosin light chain kinase.

Application

Monoclonal Anti-Myosin Light Chain Kinase recognizes the myosin light chain kinase of smooth muscle and from non-muscle cells such as cultured fibroblasts. Reactivity has been observed with myosin light chain kinase from chicken, turkey, bovine, human, mouseand pig origin. The antibody is recommended to use in various immunological techniques, including Immunoblotting (~160kDa), Immunohistochemistry and immunofluorescence.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Other Notes

This product is for R&D use only, not for drug, household, or other uses.

Storage Class

10 - Combustible liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service