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SAB4200871

Sigma-Aldrich

Anti-Pseudomonas aeruginosa Exotoxin A antibody, Mouse monoclonal

clone EXO-68, purified from hybridoma cell culture

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Synonym(s):
ETA, PE, Pseudomonas Exotoxin A

antibody form

purified from hybridoma cell culture

Quality Level

clone

EXO-68

form

liquid

species reactivity

Pseudomonas aeruginosa

concentration

~1 mg/mL

technique(s)

ELISA: 2.5-5 μg/mL using Exotoxin A from Pseudomonas aeruginosa for coating.
immunoblotting: 0.125-0.25 μg/mL using exotoxin A from Pseudomonas aeruginosa.

isotype

IgM

storage temp.

−20°C

target post-translational modification

unmodified

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This Item
P2318SAB4200866SAB4200859
clone

EXO-68

clone

polyclonal

clone

-

clone

SEB4

antibody form

purified from hybridoma cell culture

antibody form

whole antiserum

antibody form

IgG fraction of antiserum

antibody form

purified from hybridoma cell culture

technique(s)

ELISA: 2.5-5 μg/mL using Exotoxin A from Pseudomonas aeruginosa for coating., immunoblotting: 0.125-0.25 μg/mL using exotoxin A from Pseudomonas aeruginosa.

technique(s)

dot blot: 1:20,000, indirect ELISA: 1:250,000

technique(s)

immunoblotting: 1:10,000-1:20,000 using P.aeruginosa lysate, indirect ELISA: 1:5000- 1:0,000 using whole dead P.aeruginosa bacteria for coating

technique(s)

ELISA: 0.12-0.25 μg/mL using 1 μg/ml staphylococcal enterotoxin B for coating., immunoblotting: 0.5-1 μg/mL using staphylococcal enterotoxin B.

Quality Level

200

Quality Level

-

Quality Level

200

Quality Level

200

form

liquid

form

-

form

liquid

form

liquid

General description

Pseudomonas aeruginosa is a rod shaped, gram negative, monoflagellated, aerobic to facultative anaerobe bacteria which commonly inhabits soil and aqueous environments.1,2 Pseudomonas Exotoxin A (PE) is the most potent virulence factor secreted by some strains of P. aeruginosa It is composed of three structural domains, N-terminal domain (I) responsible for the toxin binding to its host cell receptor, middle domain (II) has a role in toxin translocation across the membrane, and C-terminal domain (III) which has ADP-ribosylation activity.4,5

Specificity

Monoclonal Anti-Exotoxin A from Pseudomonas aeruginosa antibody specifically recognizes Exotoxin A from Pseudomonas aeruginosa (ETA, PE) and has no cross reactivity with staphylococcal enterotoxin A and B (SEA, SEB) and cholera toxin.

Application

The antibody may be used in various immunochemical techniques including Immunoblotting (70 kDa) and ELISA.

Biochem/physiol Actions

P.aeruginosa is considered an opportunistic human pathogen mainly causing disease in immunocompromised patients. It is especially fatal in cystic fibrosis (CF) patients, but also presents a major problem in chronic wounds, burn wounds and infection of implanted biomaterials such as catheters.3The genome of P. aeruginosa encodes a vast arsenal of virulence factors such as, Type 3 secretion system (T3SS), type 4 pilli and several secreted proteases, lipases and phospholipases.1 The Exotoxin A ADP-ribosylation activity inhibits host elongation factor 2 (EF2), and protein synthesis.1 Due to its toxin ADP-ribosylation activity PE is considered as a selective agent for the elimination of specific cell populations resulting in the irreversible shut down of protein synthesis leading to cell death. To reduce the adverse effects of natural PE, mutated PE was used in several attempts to develop recombinant toxin-antibody or cytokines fusion fragments for therapeutic application including cancer immunotherapy.5-9

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog  our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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D J Hassett
Journal of bacteriology, 178(24), 7322-7325 (1996-12-01)
Pseudomonas aeruginosa produced alginate and elevated algD (encoding GDPmannose 6-dehydrogenase) transcription under strict anaerobic conditions, especially when using nitrate as a terminal electron acceptor. Purified alginate added to bacterial suspensions caused a decrease in growth, suggesting that alginate contributes to
I Pastan et al.
Science (New York, N.Y.), 254(5035), 1173-1177 (1991-11-22)
Recombinant toxins target cell surface receptors and antigens on tumor cells. They kill by mechanisms different from conventional chemotherapy, so that cross resistance to conventional chemotherapeutic agents should not be a problem. Furthermore, they are not mutagens and should not
Jie Gao et al.
Molecular cancer therapeutics, 7(10), 3399-3407 (2008-10-15)
We reported previously the development of SMFv-PE38KDEL type I mutant (PE38KDEL-I; Mut-I), a recombinant immunotoxin in which a single-chain antibody derived from mouse SM5-1 monoclonal antibody is genetically fused to PE38KDEL-I. In comparison with the SMFv-PE38KDEL wild-type, Mut-I showed improved
R Hertle et al.
Infection and immunity, 69(11), 6962-6969 (2001-10-13)
Pseudomonas aeruginosa is the major infectious agent of concern for cystic fibrosis patients. Strategies to prevent colonization by this bacterium and/or neutralize its virulence factors are clearly needed. Here we characterize a dual-function vaccine designed to generate antibodies to reduce
J Hwang et al.
Cell, 48(1), 129-136 (1987-01-16)
Pseudomonas exotoxin A is a single chain toxin with three structural domains that inhibits protein synthesis in eukaryotic cells by catalyzing ADP ribosylation of elongation factor 2. To study the function of these domains, we deleted different portions of the

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