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SAB4200874

Sigma-Aldrich

Anti-SARS-COV-2-Spike-RBD region antibody produced in rabbit

affinity isolated antibody, buffered aqueous solution

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Synonym(s):
ACE2-binding protein, Angiotensin Converting Enzyme 2 (ACE2)-binding region, COVID-19 Spike protein, Coronavirus, RBD Region, Severe acute respiratory syndrome coronavirus 2 (SARS-CoV2), Spike surface glycoprotein

antibody form

affinity isolated antibody

Quality Level

form

liquid

species reactivity

viral

concentration

~1 mg/mL

technique(s)

ELISA: 1/25-1/200 using 2 μg/mL SARS-CoV-2 RBD (Cat. No. SAE1000) for coating and 2 μg/ml ACE2-Biotin (Cat. No. SAE0171) for competition ((Neutralizing ELISA))
immunoblotting: 1:2000-1:4000 using SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating.
indirect ELISA: 1/2000-1/4000 using 2 μg/mL SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

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SAB4200873SAB4200872SAB4200889
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

-

form

liquid

form

liquid

form

liquid

form

lyophilized powder

technique(s)

ELISA: 1/25-1/200 using 2 μg/mL SARS-CoV-2 RBD (Cat. No. SAE1000) for coating and 2 μg/ml ACE2-Biotin (Cat. No. SAE0171) for competition ((Neutralizing ELISA)), indirect ELISA: 1/2000-1/4000 using 2 μg/mL SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating, immunoblotting: 1:2000-1:4000 using SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating.

technique(s)

immunoblotting: 1:2000-1:4000 using SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating., indirect ELISA: 1/2000-1/4000 using 2 μg/mL SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating

technique(s)

immunoblotting: 1:1000-1:2000 using SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating., indirect ELISA: 1/2000-1/4000 using 2 μg/mL SARS-CoV-2 Spike RBD (Cat. No. SAE1000) for coating.

technique(s)

-

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC2

General description

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) or (2019-nCoV) is a novel coronavirus that had spread on December 2019 in Hubei province of China and infected millions of people worldwide.1 The causative agent of COVID-19, the SARS-CoV-2 virus is a positive-strand RNA virus .The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S), E and M proteins help in viral assembly and N protein is needed for RNA synthesis. The main receptor for SARS-CoV and SARS-CoV-2 on the membrane of the target cells is the Angiotensin 2 Converting Enzyme (ACE2), a metallopeptidase present on the membrane of many cells, including type-I and -II pneumocytes, small intestine enterocytes, kidney proximal tubules cells, the endothelial cells of arteries and veins, and the arterial smooth muscle, among other tissues.15-16 It has been shown that SARS-CoV-2 virus employs transmembrane protease serine 2 (TMPRSS2) for S protein priming and it is speculated that furin-mediated cleavage at the S1/S2 site in infected cells may promote subsequent TMPRSS2-dependent entry into target cells.

Specificity

Anti-SARS-CoV-2-Spike-RBD region antibody specifically recognizes Spike from COVID-19 virus origin.

Immunogen

Synthetic peptide CYNG-15

Application

The antibody may be used in various immunochemical techniques including Immunoblotting and Elisa. Detection of the Spike RBD protein band by Immunoblotting is specifically inhibited by the immunogen.The antibody may be used in Neutralizing ELISA, to block the Spike RBD-ACE2 interaction

Biochem/physiol Actions

The Spike protein (S) is responsible for virus binding and entry into host cells. The S precursor protein of SARS-CoV-2 is cleaved into S1 subunit (685 amino acids), and S2 (588 amino acids) subunits. S1 subunit has the receptor binding domain (RBD) that mediates entry of this virus into susceptible cells through the peptidase domain of host ACE2 with high affinity (Kd = 15 nM). S2 protein, which is reported to be well-conserved and shows 99% identity with bat coronavirus, is responsible for membrane fusion. The Spike protein is the most studied of the coronaviruses proteins, due to his crucial role in the host cell entry , it contains the RBD for the ligand on the host cell membrane (the ACE2 protein), and also has epitopes recognized by T and B cells.

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative.

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Data presented is the available current product information and provided as-is. This product has not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Storage Class

12 - Non Combustible Liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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