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SAB4200875

Sigma-Aldrich

Anti-SARS-COV-2-Spike-RBD region antibody, Mouse monoclonal

clone SP-10, purified from hybridoma cell culture

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biological source

mouse

Quality Level

antibody form

purified from hybridoma cell culture

antibody product type

primary antibodies

clone

SP-10

form

liquid

species reactivity

viral

packaging

pkg of 100 μL
pkg of 25 μL

concentration

~1 mg/mL

technique(s)

ELISA: 0.25-0.5 μg/mL using SARS-CoV-2 RBD (Cat. No. SAE1000) for coating
immunoblotting: 0.1-0.2 μg/mL using SARS-CoV-2 RBD (Cat. No. SAE1000)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

target post-translational modification

unmodified

General description

Severe Acute Respiratory Syndrome Coronavirus 2 (SARS-CoV-2) or (2019-nCoV) is a novel coronavirus that had spread on December 2019 in Hubei province of China and infected millions of people worldwide.1 The causative agent of COVID-19, the SARS-CoV-2 virus is a positive-strand RNA virus .The mature SARS-CoV-2 contains 4 structural proteins: Envelope (E), Membrane (M), Nucleocapsid (N), and the Spike protein (S), E and M proteins help in viral assembly and N protein is needed for RNA synthesis.

Specificity

Monoclonal Anti-SARS-COV-2-Spike-RBD region antibody specifically recognizes Spike from COVID-19 virus origin

Application

The antibody may be used in various immunochemical techniques including Immunoblotting and Elisa. Detection of the Spike RBD protein band by Immunoblotting is specifically inhibited by the immunogen.

Biochem/physiol Actions

The Spike protein (S) is responsible for virus binding and entry into the host cells. SARS-CoV-2 S protein precursor is cleaved into S1 subunit (685 amino acids), and S2 (588 amino acids) subunits. S1 subunit harbor the receptor binding domain (RBD) that mediates virus entry into susceptible cells through the peptidase domain of host ACE2 with high affinity (Kd = 15 nM). S2 protein, which is reported to be well-conserved and showing 99% identity with bat coronavirus, is responsible for the membrane fusion. The Spike protein is the most studied between the coronaviruses proteins, due to its crucial role in the host cell entry , it contains the RBD for the ligand on the host cell membrane (the ACE2 protein), and also has epitopes recognized by T and B cells, which induce the production of neutralizing antibodies.2

Physical form

Supplied as a solution in 0.01 M phosphate buffered saline pH 7.4, containing 15 mM sodium azide as a preservative

Storage and Stability

For continuous use, store at 2-8°C for up to one month. For extended storage, freeze in working aliquots. Repeated freezing and thawing is not recommended. If slight turbidity occurs upon prolonged storage, clarify the solution by centrifugation before use. Working dilution samples should be discarded if not used within 12 hours.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.Data presented is the available current product information and provided as-is. This product has not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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