All Photos(2)

SAB4300102

Sigma-Aldrich

Anti-phospho-AKT2 (pSer474) antibody produced in rabbit

affinity isolated antibody

Synonym(s):
Anti-RAC-BETA antibody produced in rabbit, Anti-PKBBETA antibody produced in rabbit, Anti-PKBB antibody produced in rabbit, Anti-PRKBB antibody produced in rabbit, Anti-v-akt murine thymoma viral oncogene homolog 2 antibody produced in rabbit
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~60 kDa

species reactivity

rat, human, mouse

concentration

1 mg/mL

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
western blot: 1:500-1:1000

isotype

IgG

immunogen sequence

(Q-F-SP-Y-S)

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AKT2(208)

Immunogen

Peptide sequence around phosphorylation site of serine 474 (Q-F-S(p)-Y-S), according to the protein AKT2.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

General protein kinase capable of phosphorylating several known proteins.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Jinlong Zhao et al.
International journal of medical sciences, 15(10), 1062-1071 (2018-07-18)
Rac1, known as a "molecular switch", plays a crucial role in plenty of cellular processes. Rac1 aggravates the damage of myocardial cells in the process of myocardial ischemia-reperfusion during myocardial infarction through activating the NADPH oxidase and bringing about the...
Victoria H Cruz et al.
Molecular metabolism, 16, 139-149 (2018-06-25)
TANK Binding Kinase 1 (TBK1) has been implicated in the regulation of metabolism through studies with the drug amlexanox, an inhibitor of the IκB kinase (IKK)-related kinases. Amlexanox induced weight loss, reduced fatty liver and insulin resistance in high fat...
Alberto Bavelloni et al.
FASEB journal : official publication of the Federation of American Societies for Experimental Biology, 33(8), 9044-9061 (2019-05-17)
Murine thymoma viral oncogene homolog (AKT) kinases target both cytosolic and nuclear substrates for phosphorylation. Whereas the cytosolic substrates are known to be closely associated with the regulation of apoptosis and autophagy or metabolism and protein synthesis, the nuclear substrates...
K Soukupová et al.
Journal of trace elements in medicine and biology : organ of the Society for Minerals and Trace Elements (GMS), 52, 58-67 (2019-02-09)
The specific effects of sodium selenite (selenite) on a chemoresistant human bladder cancer cell line RT-112/D21 were investigated during 72 h. Selenite at low concentration of 2.5 μmol (otherwise tolerated in normal urothelial cells UROtsa) suppressed growth and proliferation of the tested...
Daolu Yuwen et al.
Cancer epidemiology, biomarkers & prevention : a publication of the American Association for Cancer Research, cosponsored by the American Society of Preventive Oncology, 28(1), 163-173 (2018-09-20)
Platinum-based doublets with a third-generation agent are the recommended option for many patients with non-small cell lung cancer (NSCLC) with no contraindications to platinum compounds. Unfortunately, the clinical effectiveness of such chemotherapy is limited by intrinsic or acquired resistance. Circulating...

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