SAB4300339

Sigma-Aldrich

Anti-BCL2 (Ab-56) antibody produced in rabbit

affinity isolated antibody

Synonym(s):
Anti-B-cell CLL / lymphoma 2 antibody produced in rabbit, Anti-Bcl-2 antibody produced in rabbit
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~26 kDa

species reactivity

human

concentration

1 mg/mL

application(s)

immunohistochemistry (formalin-fixed, paraffin-embedded sections): 1:50-1:100
indirect immunofluorescence: 1:100-1:200
western blot: 1:500-1:1000

isotype

IgG

immunogen sequence

(G-H-T-P-H)

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... BCL2(596)

General description

BCL2 (B-cell lymphoma 2) consists of pro- and antiapoptotic functions. It belongs to BCL2 family of proteins. It is mapped to chromosome 18q21.3 .

Immunogen

Peptide sequence around aa. 54-58 (G-H-T-P-H), according to the protein BCL2.

Application

Anti-BCL2 (Ab-56) antibody has been used in western blotting .

Biochem/physiol Actions

Overexpression of BCL2 (B-cell lymphoma 2) results in poor clinical predictions in diffuse large B-cell lymphoma (DLBCL). It plays an important role in the growth and progression of cancer.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

Suppresses apoptosis in a variety of cell systems including factor-dependent lymphohematopoietic and neural cells. Regulates cell death by controlling the mitochondrial membrane permeability. Appears to function in a feedback loop system with caspases. Inhibits caspase activity either by preventing the release of cytochrome c from the mitochondria and/or by binding to the apoptosis-activating factor (APAF-1).

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

RLIP76-dependent suppression of PI3K/AKT/Bcl-2 pathway by miR-101 induces apoptosis in prostate cancer
Yang J, et al.
Biochemical and Biophysical Research Communications (2015)
miR-217 regulates tumor growth and apoptosis by targeting the MAPK signaling pathway in colorectal cancer
Zhang N, et al.
Oncology Letters (2016)
BCL2 genotypes and prostate cancer survival
Renner W, et al.
Strahlentherapie und Onkologie : Organ der Deutschen Rontgengesellschaft ... [Et Al] (2017)
BCL2 expression in DLBCL: reappraisal of immunohistochemistry with new criteria for therapeutic biomarker evaluation
Tsuyama N, et al.
Blood (2017)
CD164 regulates proliferation and apoptosis by targeting PTEN in human glioma
Tu M, et al.
Molecular Medicine Reports (2017)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service