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SAB4300663

Sigma-Aldrich

Anti-PKM1 antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
Anti-OIP3, Anti-PK2, Anti-PK3, Anti-PKM
NACRES:
NA.41

biological source

rabbit

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

~60 kDa

species reactivity

mouse, human

concentration

1 mg/mL

technique(s)

western blot: 1:500-1:1000

isotype

IgG

immunogen sequence

(V-R-A-S-S)

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... PKM1(5315)

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This Item
SAB4502370SAB4502372SAB4502371
conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

clone

polyclonal

clone

polyclonal

clone

polyclonal

clone

polyclonal

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

mol wt

~60 kDa

mol wt

antigen 101 kDa

mol wt

antigen 101 kDa

mol wt

antigen 101 kDa

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General description

PKM1 (pyruvate kinase M1/2) is an enzyme that belongs to the pyruvate kinase (PK) family. It is upregulated in majority of the cells other than adult muscle, brain and liver. PKM has two isoforms, M1 and M2, encoded by gene located on chromosome 15q23. PKM1 can exist as a tetramer whereas PKM2 can remain as dimer and tetramer.

The antibody detects endogenous levels of total PKM1 protein.

Immunogen

Peptide sequence around aa. 399-403 (V-R-A-S-S), according to the protein NP_872270.1

Biochem/physiol Actions

Pyruvate kinase (PK) is an important enzyme in glycolysis as it helps to convert phosphoenolpyruvate (PEP) into pyruvate. Pyruvate kinase-M2 (PKM2) knockdown prevents the multiplication, movement and invasion in ovarian cancer SKOV3 and OVCAR3 cells. It plays an important role in cell survival to oxidative stress. It also participates in the development of tumor.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Target description

Glycolytic enzyme that catalyzes the transfer of a phosphoryl group from phosphoenolpyruvate (PEP) to ADP, generating ATP. Stimulates POU5F1-mediated transcriptional activation. Plays a general role in caspase independent cell death of tumor cells. The ratio betwween the highly active tetrameric form and nearly inactive dimeric form determines whether glucose carbons are channeled to biosynthetic processes or used for glycolytic ATP production. The transition between the 2 forms contributes to the control of glycolysis and is important for tumor cell proliferation and survival.

Physical form

Solution in phosphate-buffered saline containing 0.02% sodium azide and 50% glycerol

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class Code

10 - Combustible liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Inhibition of Proliferation, Migration, and Invasion by Knockdown of Pyruvate Kinase-M2 (PKM2) in Ovarian Cancer SKOV3 and OVCAR3 Cells
Miao Y, et al.
Oncology Research (2016)
PKM2, a Central Point of Regulation in Cancer Metabolism
Wong N, et al.
International Journal of Cell Biology (2013)
Enhanced expression of the M2 isoform of pyruvate kinase is involved in gastric cancer development by regulating cancer-specific metabolism
Shiroki T, et al.
Cancer Science (2017)

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