SAB4500007

Sigma-Aldrich

Anti-AKT1 antibody produced in rabbit

affinity isolated antibody

Synonym(s):
Protein kinase Akt-2, PKB, Protein kinase B, C-AKT/ RAC-PK-β, RAC-PK-α
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

application(s)

ELISA: 1:40000
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AKT1(207)

General description

The AKT1 (AKT serine/threonine kinase 1) gene is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα (protein kinase B). It is upregulated in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker and a carboxyl-terminal kinase domain.

Anti-AKT1 Antibody detects endogenous levels of total AKT1 protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human AKT1/2/3.

Immunogen Range: 281-330

Biochem/physiol Actions

The protein encoded by AKT1 (AKT serine/threonine kinase 1) gene controls the survival of cell and anti-apoptotic actions that attack the pathogenesis of several cancers, hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

AKT plays a central role in tumorigenesis
Testa JR and Bellacosa A
Proceedings of the National Academy of Sciences of the USA (2001)
Association between single nucleotide polymorphisms in AKT1 and the risk of prostate cancer in the Chinese Han population
Liu JM, et al.
Genetics and molecular research : GMR (2017)
Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice
Cho H, et al.
The Journal of Biological Chemistry (2001)
Tetsuo Mashima et al.
Cancer research, 74(17), 4888-4897 (2014-06-26)
Endocrine therapy is the standard treatment for advanced prostate cancer; however, relapse occurs in most patients with few treatment options available after recurrence. To overcome this therapeutic hurdle, the identification of new molecular targets is a critical issue. The capability...
Hua-Yu Zhu et al.
PloS one, 9(5), e97114-e97114 (2014-05-13)
As an important oncogenic miRNA, microRNA-21 (miR-21) is associated with various malignant diseases. However, the precise biological function of miR-21 and its molecular mechanism in hypertrophic scar fibroblast cells has not been fully elucidated. Quantitative Real-Time PCR (qRT-PCR) analysis revealed...

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service