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SAB4500487

Sigma-Aldrich

Anti-PARP (Cleaved-Asp214) antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
ADP-ribosyltransferase 1, ADPRT, ADPRT1, PARP, pADPRT-1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 24 kDa

species reactivity

mouse, human

concentration

~1 mg/mL

technique(s)

ELISA: 1:20000
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

proteolytically cleaved (Asp214)

Gene Information

human ... PARP1(142)

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SAB4500488SAB1403527WH0000142M1
antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

purified immunoglobulin

antibody form

purified immunoglobulin

Quality Level

100

Quality Level

100

Quality Level

100

Quality Level

100

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

form

buffered aqueous solution

technique(s)

ELISA: 1:20000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:1000, western blot: 1:500-1:1000

technique(s)

capture ELISA: suitable, immunofluorescence: suitable, indirect ELISA: suitable, western blot: 1-5 μg/mL

technique(s)

indirect ELISA: suitable, indirect immunofluorescence: suitable, western blot: 1-5 μg/mL

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General description

Anti-PARP (Cleaved-Asp214) Antibody detects endogenous levels of fragment of activated PARP (Cleaved-Asp214) protein.
PARP1 (poly (ADP-ribose) polymerase 1) is a nuclear protein that consists of three functional domains. It belongs to the PARP family. It is located on chromosome 1q42.

Immunogen

The antiserum was produced against synthesized peptide derived from human PARP.

Immunogen Range: 165-214

Application

Anti-PARP (Cleaved-Asp214) antibody has been used in Western Blotting.

Biochem/physiol Actions

PARP1 (poly (ADP-ribose) polymerase 1) participates in DNA repair. It serves as a facilitator of homologous recombination. It controls transcription by regulating chromatin structure, altering DNA methylation patterns, acting as a co-regulator of transcription factors and interacting with chromatin insulators. It also plays an important role in the protection of cardiovascular system.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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PARP-1 protein expression in glioblastoma multiforme
Galia A, et al.
European Journal of Histochemistry, 56 (2012)
Trapping of PARP1 and PARP2 by Clinical PARP Inhibitors
Murai J, et al.
Cancer Research, 72 (2012)
Fascin2 regulates cisplatin-induced apoptosis in NRK-52E cells
Wang X, et al.
Toxicology Letters (2017)
Loss of ?(E)-catenin potentiates cisplatin-induced nephrotoxicity via increasing apoptosis in renal tubular epithelial cells
Wang X, et al.
Toxicological Sciences, 141 (2014)
Huajun Xi et al.
Molecular medicine reports, 24(3) (2021-07-20)
Forkhead‑box gene 1 (FOXG1) has been reported to serve an important role in various malignancies, but its effects on nasopharyngeal cancer (NPC) remain unknown. Thus, the present study aimed to investigate the specific regulatory relationship between FOXG1 and NPC progression. Tumor tissues and

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