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SAB4500713

Sigma-Aldrich

Anti-EGFR antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
ERBB1, Epidermal growth factor receptor, Epidermal growth factor receptor precursor, Proto-oncogene c-ErbB-1, Receptor tyrosine-protein kinase erbB-1
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 134 kDa

species reactivity

human, rat, mouse

concentration

~1 mg/mL

technique(s)

ELISA: 1:5000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... EGFR(1956)

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SAB4500721SAB4500718SAB4500714
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

mol wt

antigen 134 kDa

mol wt

antigen 134 kDa

mol wt

antigen 134 kDa

mol wt

antigen 134 kDa

UniProt accession no.

P00533

UniProt accession no.

P00533

UniProt accession no.

P00533

UniProt accession no.

P00533

technique(s)

ELISA: 1:5000, immunohistochemistry: 1:50-1:100, immunofluorescence: 1:100-1:500, western blot: 1:500-1:1000

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: 1:40000, western blot: 1:500-1:1000

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General description

Epidermal growth factor receptor (EGFR, ErbB1) is a transmembrane protein that exerts tyrosine kinase activity upon ligand induced activation. EGFR can be activated by binding EGF or at least six other structurally related protein ligands, including transforming growth factor-α (TGF-α), heparin-binding EGF-like growth factor (HB-EGF), betacellulin (BTC), amphiregulin, epiregulin and epigen. The gene encoding it is localized on human chromosome 7p11.2. Anti-EGFR Antibody detects endogenous levels of total EGFR protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human EGFR.

Immunogen Range: 986-1035

Biochem/physiol Actions

Upon activation by ligands, epidermal growth factor receptor (EGFR, ErbB1) initiates a signaling cascade which includes dimerization and internalization, tyrosine phosphorylation, DNA synthesis of target genes, and, ultimately, cell proliferation. EGFR signaling plays a role in the growth and differentiation of normal cells, but elevated EGFR activity is correlated with the development and pathogenesis of certain cancers.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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A chromosomal region 7p11.2 transcript map: its development and application to the study of EGFR amplicons in glioblastoma.
Eley GD
Neuro-Oncology, 4(2), 86-94 (2002)
Molecular mechanisms of resistance to the EGFR monoclonal antibody cetuximab.
Brand TM
Cancer Biology & Therapy, 11(9), 777-792 (2011)

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