SAB4500797

Sigma-Aldrich

Anti-AKT antibody produced in rabbit

affinity isolated antibody

Synonym(s):
AKT1 kinase, AKT, PKB, kinase Akt1, C-AKT
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

rat, human, mouse

concentration

~1 mg/mL

application(s)

ELISA: 1:1000
western blot: 1:500-1:1000

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AKT1(207)

General description

Anti-AKT Antibody detects endogenous levels of total AKT protein.
AKT serine/threonine kinase 1 (AKT1), also called as v-akt murine thymoma viral oncogene homolog 1 or protein kinase B α (PKB-α), is encoded by the gene mapped to human chromosome 14q32. It is highly expressed in liver, muscle and adipocytes. The encoded protein is a member of the AKT family, which is characterized with an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker and a carboxyl-terminal kinase domain.

Immunogen

The antiserum was produced against synthesized peptide derived from human Akt.

Immunogen Range: 90-139

Application

Anti-AKT antibody produced in rabbit has been used in Western Blotting.

Biochem/physiol Actions

AKT serine/threonine kinase 1 (AKT1) acts as a regulator of cell-survival and anti-apoptotic functions. This protein has a crucial role in tumorigenesis. Mutations in the gene are associated with the pathogenesis of prostate cancer (PC). Elevated expression of the gene has been observed in human gastric cancer. Aberrations in AKT1 signaling leads to the development of schizophrenia.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

AKT plays a central role in tumorigenesis
Joseph R
Proceedings of the National Academy of Sciences of the USA (2001)
AKT plays a central role in tumorigenesis
Effat S
Nature Genetics (2004)
PBX3 is associated with proliferation and poor prognosis in patients with cervical cancer
Hongfang Li
OncoTargets and Therapy (2017)
Effect of Momordica charantia protein on proliferation, apoptosis and the AKT signal transduction pathway in the human endometrial carcinoma Ishikawa H cell line in vitro
Hang -Zhi Chu
Oncology Letters (2017)
Akt1/PKBa Is Required for Normal Growth but Dispensable for Maintenance of Glucose Homeostasis in Mice*
Han Cho
The Journal of Biological Chemistry (2001)

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