SAB4500802

Sigma-Aldrich

Anti-AKT antibody produced in rabbit

affinity isolated antibody

Synonym(s):
AKT1 kinase, AKT, PKB, kinase Akt1, C-AKT
MDL number:
NACRES:
NA.41

biological source

rabbit

Quality Level

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 55 kDa

species reactivity

mouse, rat, human

concentration

~1 mg/mL

application(s)

ELISA: 1:20000
immunofluorescence: 1:100-1:500
immunohistochemistry: 1:50-1:100
western blot: 1:500-1:1000

conjugate

unconjugated

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

human ... AKT1(207)

General description

AKT1 (AKT serine/threonine kinase 1) is also called as v-akt murine thymoma viral oncogene homolog 1 and PKBα(protein kinase B). It is upregulated in liver, muscle and adipocytes. AKT1 gene is mapped to human chromosome 14q32. It is a member of AKT family that has an amino-terminal pleckstrin homology (PH) domain, a short α-helical linker and a carboxyl-terminal kinase domain.
Anti-AKT Antibody detects endogenous levels of total AKT protein.

Immunogen

The antiserum was produced against synthesized peptide derived from human Akt.

Immunogen Range: 292-341

Application

Anti-AKT1 (Ab-473) antibody has been used in western blotting and immunostaining.

Biochem/physiol Actions

AKT1 (AKT serine/threonine kinase 1) controls the survival of cell and anti-apoptotic actions that attack the pathogenesis of several cancers , hence it plays a crucial role in tumorigenesis. In mice, AKT1 is essential for normal development.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

storage_class_code

12 - Non Combustible Liquids

WGK Germany

nwg

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis

Certificate of Origin

Akt1/PKBalpha is required for normal growth but dispensable for maintenance of glucose homeostasis in mice
Cho H, et al.
The Journal of Biological Chemistry (2001)
Association between single nucleotide polymorphisms in AKT1 and the risk of prostate cancer in the Chinese Han population
Liu JM, et al.
Genetics and molecular research : GMR (2017)
AKT plays a central role in tumorigenesis
Testa JR and Bellacosa A
Proceedings of the National Academy of Sciences of the USA (2001)
Dysregulation of microRNAs and renin-angiotensin system in high salt diet-induced cardiac dysfunction in uninephrectomized rats
Amara VR, et al.
PLoS ONE (2017)
ZP3 is Required for Germinal Vesicle Breakdown in Mouse Oocyte Meiosis
Gao LL, et al.
Scientific Reports (2017)

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