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SAB4503081

Sigma-Aldrich

Anti-Vimentin antibody produced in rabbit

affinity isolated antibody

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Synonym(s):
VIME, vimentin
NACRES:
NA.41

biological source

rabbit

Quality Level

conjugate

unconjugated

antibody form

affinity isolated antibody

antibody product type

primary antibodies

clone

polyclonal

form

buffered aqueous solution

mol wt

antigen 53 kDa

species reactivity

human, mouse, rat

concentration

~1 mg/mL

technique(s)

ELISA: 1:10000
western blot: 1:500-1:1000

NCBI accession no.

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

target post-translational modification

unmodified

Gene Information

human ... VIM(7431)

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SAB4503082SAB4503083SAB1305445
biological source

rabbit

biological source

rabbit

biological source

rabbit

biological source

rabbit

conjugate

unconjugated

conjugate

unconjugated

conjugate

unconjugated

conjugate

-

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

antibody form

affinity isolated antibody

Gene Information

human ... VIM(7431)

Gene Information

human ... VIM(7431)

Gene Information

human ... VIM(7431)

Gene Information

human ... VIM(7431)

technique(s)

ELISA: 1:10000, western blot: 1:500-1:1000

technique(s)

ELISA: 1:20000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

ELISA: 1:10000, immunohistochemistry: 1:50-1:100, western blot: 1:500-1:1000

technique(s)

flow cytometry: 1:50, immunofluorescence: 1:50, immunohistochemistry: 1:500, western blot: 1:4000

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General description

Anti-Vimentin Antibody detects endogenous levels of total Vimentin protein.
The VIM (vimentin) gene codes for an intermediate filament protein, that is expressed throughout mesenchymal cells. VIM gene is mapped to human chromosome 10p13.

Immunogen

The antiserum was produced against synthesized peptide derived from human Vimentin.

Immunogen Range: 56-105

Biochem/physiol Actions

Vimentin is known to promote epithelial to mesenchymal transition and contributes to metastasis in cancer such as ovarian cancer. Vimentin preserves cellular structure and tissue integrity. Vimentin is also known to bring about TNF-α (tumor necrosis factor-α) induced cell apoptosis.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Rabbit IgG in phosphate buffered saline (without Mg2+ and Ca2+), pH 7.4, 150mM NaCl, 0.02% sodium azide and 50% glycerol.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

10 - Combustible liquids

wgk_germany

nwg

flash_point_f

Not applicable

flash_point_c

Not applicable


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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

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Both RhoA/ROCK and Raf-1/CK2 pathway play essential roles in cell proliferation, apoptosis, differentiation, and multiple other common cellular functions. We previously reported that vimentin is responsible for TNF-α-induced cell apoptosis. Herein, we investigated the regulation of RhoA/ROCK and Raf-1/CK2 signaling

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