SAB4600031

Sigma-Aldrich

Anti-Chicken IgY (H+L), highly cross-adsorbed, CF 488A antibody produced in donkey

~2 mg/mL, affinity isolated antibody

NACRES:
NA.46
Pricing and availability is not currently available.

Quality Level

biological source

donkey

antibody form

affinity isolated antibody

antibody product type

secondary antibodies

clone

polyclonal

form

buffered aqueous solution

species reactivity

chicken

concentration

~2 mg/mL

application(s)

flow cytometry: 1-10 μg/mL
immunocytochemistry: suitable
immunohistochemistry: suitable
indirect immunofluorescence: 1-10 μg/mL

fluorescence

λex 490 nm; λem 515 nm

conjugate

CF 488A conjugate

shipped in

dry ice

storage temp.

−20°C

Related Categories

General description

Immunoglobulin Y is one of the antibodies produced in birds. It is generally related to mammalian IgG. However, IgY has different characteristics compared to mammalian IgG, for example, IgY does not bind with mammalian complements nor Rheumatoid factor, which are present in mammalian serum and anti-mammalian IgG antibodies. IgG antibodies, which often cross-react to IgG from different mammalian species, exhibit low cross-reactivity to IgY. These characteristics of IgY is beneficial when it is used as a biochemical/clinical reagent. Immunoglobulin Y present in chicken blood serum has the capability to pass across the follicular epithelium of the ovary and aggregate in the yolk during oogenesis. Structurally, IgY and mammalian IgG differ in the heavy chain with the molecular weight of 68,000 and 50,000 dalton, respectively. Mammalian IgG has slightly higher stability compared to IgY.
CF 488A is a green fluorescent dye with excitation argon laser line at 488nm. CF 488A is minimally charged, thus, it reduces non-specific binding of antibody conjugates. CF 488A is a highly water-soluble and pH-insensitive dye with molecular weight of ~710. This dye has absorption/emission maxima at 490/515nm and it is highly photostable.

Specificity

Binds all chicken IgYs (minimal cross-reaction with bovine, goat, guinea pig, syrian hamster, horse, human, mouse, rabbit, rat and sheep proteins)

Immunogen

chicken IgY (H+L)

Application

Anti-Chicken IgY (H+L), highly cross-adsorbed, CF 488A antibody produced in donkey has been used in immunohistochemistry.

Features and Benefits

Evaluate our antibodies with complete peace of mind. If the antibody does not perform in your application, we will issue a full credit or replacement antibody. Learn more.

Physical form

Supplied in phosphate buffered saline with 0.05% sodium azide, 50% glycerol and 2 mg/mL bovine serum albumin.

Preparation Note

Protect from light.

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Certificate of Analysis
Certificate of Origin
Nina Hellström Erkenstam et al.
Frontiers in cellular neuroscience, 10, 286-286 (2016-12-27)
Immune cells display a high degree of phenotypic plasticity, which may facilitate their participation in both the progression and resolution of injury-induced inflammation. The purpose of this study was to investigate the temporal expression of genes associated with classical and...
Temporal Characterization of Microglia/Macrophage Phenotypes in a Mouse Model of Neonatal Hypoxic-Ischemic Brain Injury.
Hellstrom Erkenstam N
Frontiers in Cellular Neuroscience, 10, 286-286 (2016)
Jiwei Yao et al.
Nature neuroscience, 21(11), 1541-1550 (2018-10-27)
Urination (also called micturition) is thought to be regulated by a neural network that is distributed in both subcortical and cortical regions. Previously, urination-related neurons have been identified in subcortical structures such as the pontine micturition center (also known as...
Jiwei Yao et al.
Frontiers in neuroscience, 13, 663-663 (2019-07-12)
Understanding the complex neural mechanisms controlling urinary bladder activity is an extremely important topic in both neuroscience and urology. Simultaneously recording of the bladder activity and neural activity in related brain regions will largely advance this field. However, such recording...
Leah J Greenspan et al.
Cell reports, 24(13), 3466-3476 (2018-09-27)
Homeostasis in adult tissues depends on the precise regulation of stem cells and their surrounding microenvironments, or niches. Here, we show that the cell cycle inhibitor and tumor suppressor Retinoblastoma (RB) is a critical regulator of niche cells in the...

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