SAB5700588

Sigma-Aldrich

Monoclonal Anti-2019-nCoV N Protein antibody produced in Rabbit

Synonym(s):
Nucleoprotein

biological source

rabbit

Quality Level

antibody product type

primary antibodies

clone

monoclonal

form

liquid

species reactivity

SARS virus

application(s)

ELISA: 1:1000-1:5000
flow cytometry: 1:50-1:200
immunofluorescence: 1:50-1:200
immunohistochemistry: 1:50-1:200
immunoprecipitation (IP): 1:50-1:200
western blot: 1:1000-1:5000

isotype

IgG

conjugate

unconjugated

NCBI accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

SARS virus ... N(1489678)

General description

Coronaviruses are enveloped viruses with a positive-sense RNA genome and with a nucleocapsid of helical symmetry. Coronavirus nucleoproteins localize to the cytoplasm and the nucleolus, a subnuclear structure, in both virus-infected primary cells and in cells transfected with plasmids that express N protein. Coronavirus N protein is required for coronavirus RNA synthesis, and has RNA chaperone activity that may be involved in template switch. Nucleocapsid protein is a most abundant protein of coronavirus. During virion assembly, N protein binds to viral RNA and leads to formation of the helical nucleocapsid. Nucleocapsid protein is a highly immunogenic phosphoprotein also implicated in viral genome replication and in modulating cell signaling pathways. Because of the conservation of N protein sequence and its strong immunogenicity, the N protein of coronavirus is chosen as a diagnostic tool.

Storage and Stability

Avoid freeze / thaw cycles. Buffer:PBS with 0.02% sodium azide, 50% glycerol, pH 7.3.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

WGK Germany

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificate of Analysis
Certificate of Origin

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