SAB5700591

Recombinant 2019-nCoV Spike S1 Protein with hFc and His tag

• Synonym(s): S glycoprotein Subunit1, S1 protein, Spike glycoprotein Subunit1, Spike protein S1, novel coronavirus s1 Protein
• NACRES: NA.26
• UNSPSC Code: 12352203
• Form: lyophilized powder
• Assay: >95% (SDS-PAGE)
• Recombinant: expressed in HEK 293 cells
• Mol wt: 103 kDa

Properties

• recombinant: expressed in HEK 293 cells
• assay: >95% (SDS-PAGE)
• form: lyophilized powder
• mol wt: 103 kDa
• impurities: <0.1 EU/μg protein (LAL test)
• UniProt accession no.: P0DTC2
• shipped in: wet ice
• storage temp.: −20°C
• Quality Level: 100
• Gene Information: SARS virus ... S(43740568)

Description

General description

The spike protein (S) of coronavirus (CoV) attaches the virus to its cellular receptor, angiotensin-converting enzyme 2 (ACE2). A defined receptor-binding domain (RBD) on S mediates thisinteraction.The S protein plays key parts in the induction of neutralizing-antibody and T-cellresponses, as well as protective immunity.
Recombinant 2019-nCoV Spike S1 Protein is produced by HEK293 cells expression system. The target protein is expressed with sequence (Val11-Arg682) of 2019-ncov Spike S1 (Accession #YP_009724390.1) fused with an Fc, 6×His tag at the C-terminus.

Biochem/physiol Actions

Measured by its binding ability in a functional ELISA. Immobilized Recombinant Human ACE2 at 2μg/mL (100 μL/well) can bind Recombinant SARS-COV-2 Spike S1, the EC50 of SARS-COV-2 Spike S1 is 6.79 ng/mL. Immobilized Human ACE2 on COOH Chip, can bind SARS-COV-2 Spike S1 with an affinity constant of 90.8 nM as determined in a SPR assay.

Preparation Note

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Safety Information

• Storage Class: 11 - Combustible Solids
• wgk: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable