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SAB5700596

Sigma-Aldrich

Recombinant 2019-nCoV Nucleocapsid Protein with His tag

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Synonym(s):
Nucleocapsid protein, Nucleoprotein, Protein N

recombinant

expressed in E. coli

Quality Level

Assay

>95% (HPLC)
>95% (SDS-PAGE)

form

liquid

mol wt

49.4 kDa

impurities

<1.0 EU/μg protein (LAL test)

UniProt accession no.

shipped in

wet ice

storage temp.

−20°C

Gene Information

SARS virus ... N(43740575)

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This Item
S9995SAB5700597SAB5700598
assay

>95% (HPLC), >95% (SDS-PAGE)

assay

95% (SDS-PAGE)

assay

>90% (SDS-PAGE)

assay

>95% (SDS-PAGE)

form

liquid

form

frozen liquid

form

lyophilized powder

form

liquid

mol wt

49.4 kDa

mol wt

~48 kDa

mol wt

58.8 kDa

mol wt

36.9 kDa

impurities

<1.0 EU/μg protein (LAL test)

impurities

-

impurities

<1.0 EU/μg (LAL test), <1.0 EU/μg protein (LAL test)

impurities

<1.0 EU/μg protein (LAL test)

UniProt accession no.

P0DTC9

UniProt accession no.

-

UniProt accession no.

P0DTC2

UniProt accession no.

P0DTC1

General description

Recombinant SARS-COV-2(2019-nCoV) Nucleocapsid Protein is produced by E. coli expression system. The target protein is expressed with sequence (Met1-Ala419) of SARS-COV-2(2019-nCoV) Nucleocapsid (Accession # QHD43423.2) fused with an 6XHis tag at the N-terminus.

Biochem/physiol Actions

Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-COV-2 Nucleocapsid at 2μg/mL (100 μL/well) can bind Anti-Nucleocapsid antibody with a linear range of 0.12-1.64 ng/mL. Measured by its binding ability in a functional ELISA. Immobilized Recombinant SARS-CoV-2 envelope Protein at 2μg/mL (100 μL/well) can bind Recombinant SARS-CoV-2 Nucleocapsid protein, the EC50 of Recombinant SARS-CoV-2 Nucleocapsid protein is 41.10 ng/mL.

Reconstitution

For lyophilized protein : Reconstitute to a concentration of 0.1-0.5 mg/mL in sterile distilled water.

Storage and Stability

Store the lyophilized protein at -20°C to -80°C for long term. After reconstitution, the protein solution is stable at -20°C for 3 months, at 2-8 °C for up to 1 week. This product is stable at ≤ -70°C for up to 6 months from the date of receipt. For optimal storage, aliquot into smaller quantities after centrifugation and store at recommended temperature.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Data presented is the available current product information and provided as-is. These products have not been tested or verified in any additional applications, sample types, including any clinical use. Experimental conditions must be empirically derived by the user. Our Antibody Guarantee only covers tested applications stated herein and conditions presented in our product information and is not extended to publications.

Storage Class Code

10 - Combustible liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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