SAB5801064

Alpaca Anti-Goat IgG(Fcγ) single domain Antibody

recombinant Alpaca(VHH) Nano antibody

• Synonym(s): Recombinant Alpaca single variable domain secondary Nanobody
• UNSPSC Code: 12352203
• NACRES: NA.41

Properties

• biological source: alpaca
• recombinant: expressed in E. coli
• conjugate: unconjugated (6*his tag and one cys were added at the C terminal of the VHH)
• antibody form: affinity purified immunoglobulin
• antibody product type: secondary antibodies
• clone: recombinant monoclonal
• form: lyophilized solid
• mol wt: 14 kDa
• species reactivity: goat, Alpaca
• technique(s): ELISA: 1:5000-1:20000; western blot: 1:5000-1:20000
• size: 0.1 mL
• color: colorless
• isotype: VHH
• shipped in: wet ice
• storage temp.: 2-8°C

Description

General description

Alpaca Anti-Goat IgG(Fcγ) single domain Antibody is designed to specifically detect Fc region of goat IgG. It is a monovalent, single domain, recombinant antibody derived from the variable region of heavy chain of Alpaca pacous. Based on immunoelectrophoresis and/or ELISA, Alpaca Anti-Goat IgG(Fcγ) single domain Antibody detects the Fc region of goat IgG selectively but not with mouse, rabbit, human, cynomolgus, rat IgG.

Goat antibodies are commonly used in biotechnology. They are used to prepare diagnostic reagents of immunochemical techniques. Goat IgG molecule possesses both the Fc region and the Fab region, which possessing the epitope-recognition site. The IgG contains two heavy and light chains. The heavy chain is about 50 KD and the light chain is about 25 KD. The common IgG is monomeric with a molecular weight of approximately 150 kDa.
VHH are single-domain antibodies derived from the variable regions of heavy chain of Camelidae immunoglobulin. The size of VHH is extremely small( less than15KDa) compared to other forms of antibody fragment, which significantly increase the permeability of VHH. Thus VHH is considered of great value for research, diagnostics and therapeutics.

Immunogen

Recombinant Alpaca single variable domain secondary nano antibody

Biochem/physiol Actions

Fc region of goat IgG
Isotype: VHH domain of alpaca IgG2b/2c

Packaging

100 μg in Glass bottle

Physical form

Lyophilized from 10mM PBS (pH 7.5), 0.05% sucrose, 0.1% trehalose

Preparation Note

Add 100uL dH2O or 100uL dH2O with 50% Glycerol to a final concentration of 1mg/mL

After reconstitution, store at –20 °C(Avoid freeze / thaw cycles). Stable for 12 months at -20°C

Other Notes

No cross-reactivity with mouse, rabbit, human, cynomolgus, rat IgG

Safety Information

• pictograms: GHS07
• signalword: Warning
• hcodes: H317,H412
• pcodes: P261 - P272 - P273 - P280 - P302 + P352 - P333 + P313
• Hazard Classifications: Aquatic Chronic 3 - Skin Sens. 1
• Storage Class: 13 - Non Combustible Solids
• wgk_germany: WGK 3
• flash_point_f: Not applicable
• flash_point_c: Not applicable