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SAE0023

Sigma-Aldrich

Alanine Racemase from Bacillus stearothermophilus

buffered aqueous solution, ≥10 IU/mg solid

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Synonym(s):
Alanine Racemase from Bacillus stearothermophilus
CAS Number:
Enzyme Commission number:
MDL number:
NACRES:
NA.54

biological source

Bacillus sp. (B. stearothermophilus)

Quality Level

form

buffered aqueous solution

specific activity

≥10 IU/mg solid

mol wt

Mr 78 kDa (2 subunits 39 kDa each)

storage temp.

−20°C

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This Item
A7653P5380P1903
specific activity

≥10 IU/mg solid

specific activity

~30 units/mg protein (Lowry)

specific activity

7-15 units/mg solid

specific activity

100-300 units/mg protein

form

buffered aqueous solution

form

buffered aqueous glycerol solution

form

lyophilized powder

form

lyophilized powder

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

2-8°C

mol wt

Mr 78 kDa (2 subunits 39 kDa each)

mol wt

-

mol wt

27 kDa

mol wt

-

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

-

Application

Alanine racemase is used to convert L-alanine into D-alanine. Alanine racemase, from Sigma, has been used to isomerize L-[U-14C]alanine to a racemic mixture of L/D- [14C]alanine †.

Biochem/physiol Actions

Alanine racemase is involved in alanine, aspartate and D-alanine metabolism. 3-Fluoro-D-alanine and D-Cycloserine are known to inhibit alanine racemase.† Alanine racemase monomer is composed of two domains, an eight-stranded α/β barrel at the N-terminus and a C-terminal domain. The N-terminus includes residues 1-240, whereas the C-terminal comprises of the β-strand (residues 241-388). One molecule of pyridoxalphosphate (PLP) is present as the cofactor in each subunit.†

Unit Definition

One unit will convert 1.0 μmole of D-alanine to L-alanine per minute at pH 10.5 at 30 °C in a coupled assay system with L-alanine dehydrogenase.

Physical form

Aqueous solution containing 10 mM potassium phosphate

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable


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