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SAE0045

Sigma-Aldrich

HRV-3C Protease.

N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

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Synonym(s):
Human Rhinovirus 3C Protease, Levlfqgp site protease, PreScission Protease
NACRES:
NA.54

Quality Level

Assay

≥90% (SDS-PAGE)

form

aqueous solution

mol wt

21 kDa

concentration

0.8-1.2 mg/mL

shipped in

dry ice

storage temp.

−20°C

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This Item
T4455SAE0172SAE0067
HRV-3C Protease. N-Terminal His tagged recombinant protein, aqueous solution, 0.8-1.2 mg/mL

Sigma-Aldrich

SAE0045

HRV-3C Protease.

TEV Protease

Sigma-Aldrich

T4455

TEV Protease

SUMO Protease His tagged recombinant protein, lyophilized powder

Sigma-Aldrich

SAE0067

SUMO Protease

form

aqueous solution

form

solution

form

lyophilized

form

lyophilized powder

mol wt

21 kDa

mol wt

27 kDa

mol wt

-

mol wt

27 kDa

concentration

0.8-1.2 mg/mL

concentration

-

concentration

-

concentration

-

shipped in

dry ice

shipped in

wet ice

shipped in

wet ice

shipped in

ambient

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

General description

HRV-3C protease from human rhinovirus type 14 is a protease that specifically cleaves within an eight-residue recognition sequence.
Proteolytic cleavage occurs between the Gln and Gly residues.
HRV-3C protease is a useful tool to cleave recombinant proteins that are expressed as a fusion protein with this sequence, between the carrier domain and the protein of interest.
This recombinant version contains a six-histidine tag and can be easily removed by IMAC chromatography.

Application

HRV-3C Protease has been used to remove the GST tag from the DsCystatin protein.

Unit Definition

One unit of HRV-3C Protease is defined as the amount of enzyme needed to digest 1nmole of the substrate peptide per hour (H-Glu-Ala-Leu-Phe-Gln-pNA) at 0 °C, in a reaction buffer consisting of 25 mM HEPES (pH 7.5), 150 mM NaCl, 1 mM EDTA, and 1 mM DTT.

Physical form

Supplied in a solution containing 50 mM Tris HCl (pH 7.5), 0.15 M NaCl, 1 mM TCEP and 50% (V/V) glycerol.

Storage Class Code

10 - Combustible liquids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


Certificates of Analysis (COA)

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T1503
Product Number
-
25G
Pack Size/Quantity

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705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

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Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

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Proteases for biotinylated tag removal for protein purification workflows with related reagents and technical resources.

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