MilliporeSigma
All Photos(2)

Documents

SAE0071

Sigma-Aldrich

HIV-1 GP120 protein

recombinant, expressed in HEK 293 cells

All Photos(2)
Synonym(s):
Glycoprotein 120 (gp120), Surface protein gp120
NACRES:
NA.32

recombinant

expressed in HEK 293 cells

Quality Level

200

description

low endotoxin

Assay

≥95% (SDS-PAGE)

form

lyophilized powder

mol wt

calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation)

impurities

≤1 EU/μg protein endotoxin

UniProt accession no.

P04578

storage temp.

−20°C

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAE0076SAE0078SAE0093
HIV-1 GP120 protein recombinant, expressed in HEK 293 cells

Sigma-Aldrich

SAE0071

HIV-1 GP120 protein

Tau-441 human recombinant, lyophilized powder, expressed in HEK 293 cells, ≥95% (SDS-PAGE)

Sigma-Aldrich

SAE0076

Tau-441 human

MMP-9 pre-activated human recombinant, ≥1,300 pmol/min/μg, expressed in HEK 293 cells

Sigma-Aldrich

SAE0078

MMP-9 pre-activated human

Beta-1,4-galactosyltransferase 1 B4GALT1 human recombinant, expressed in HEK 293 cells, 2000 units/mg protein

Sigma-Aldrich

SAE0093

Beta-1,4-galactosyltransferase 1

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

≥95% (SDS-PAGE)

assay

95% (SDS-PAGE)

form

lyophilized powder

form

lyophilized powder

form

liquid

form

lyophilized powder

mol wt

calculated mol wt 55 kDa (The protein migrates as a 100-130 kDa protein on SDS-PAGE due to glycosylation)

mol wt

calculated mol wt 50.3 kDa (including c-terminal His and V5 tags), observed mol wt 70-100 kDa by SDS-PAGE (The protein migrates as a 70-110 kDa protein on SDS-PAGE due to glycosylation)

mol wt

calculated mol wt 66 kDa, observed mol wt 82 kDa by SDS-PAGE (The protein migrates as a 82 kDa protein on SDS-PAGE due to glycosylation)

mol wt

-

impurities

≤1 EU/μg protein endotoxin

impurities

-

impurities

-

impurities

-

UniProt accession no.

P04578

UniProt accession no.

P10636-8

UniProt accession no.

P14780

UniProt accession no.

-

General description

Recombinant human glycoprotein 120 (gp120) is expressed in human HEK 293 cells as an C-terminally his-tagged glycoprotein with a calculated molecular mass of 55 kDa (amino acids Lys33-Arg511, with a C-terminal 8-His tag). The DTT-reduced protein migrates as a 100-130 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding.

Biochem/physiol Actions

Envelope glycoprotein GP120 (or gp120) is a glycoprotein exposed on the surface of the HIV envelope. It was discovered by Tun-Hou Lee and Myron Essex of the Harvard School of Public Health in 1988. The protein′s name is derived from its apparent molecular weight of 120 kDa. The gp120 protein is essential for virus entry into cells as it plays a vital role in attachment to specific cell surface receptors. These receptors are DC-SIGN, Heparan Sulfate Proteoglycan and the CD4 receptor. The presence of gp120 is associated with higher levels of plasma IL-6, IL-10, and TNF-α, which may contribute to immune dysfunction during early HIV infection.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Storage Class Code

11 - Combustible Solids

WGK

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (Example: T1503).

Example:

T1503
Product Number
-
25G
Pack Size/Quantity

Additional examples:

705578-5MG-PW

PL860-CGA/SHF-1EA

MMYOMAG-74K-13

1000309185

enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Lichao Peng et al.
Frontiers in neuroscience, 15, 663962-663962 (2021-07-31)
Human immunodeficiency virus envelope glycoprotein 120 (gp120) leads to hyperalgesia. Long non-coding RNAs are characterized by the lack of a protein-coding sequence and may contribute to the development and maintenance of inflammatory and neuroinflammatory pain. Rats with neuroinflammatory pain were

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service