All Photos(3)




Angiotensin Converting Enzyme, ACE, human

recombinant, ≥10 U/mg, expressed in HEK 293 cells

Sign Into View Organizational & Contract Pricing

ACE, ACE1, CD143, DCP, DCP1, Dipeptidyl carboxypeptidase I, Kininase II, Peptidyl-dipeptidase A, Peptidyldipeptide Hydrolase
Enzyme Commission number:


expressed in HEK 293 cells

Quality Level


≥95% (SDS-PAGE)


lyophilized powder

specific activity

≥10 U/mg

mol wt

calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

UniProt accession no.

shipped in

dry ice

storage temp.


General description

Recombinant human Angiotensin Converting Enzyme (ACE) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 138 kDa (amino acids 30-1232). The DTT-reduced protein migrates as a 180 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.
The ACE gene codes for angiotensin-converting enzyme, which is one of the major enzymes in the RAS (renin angiotensin system). The ACE gene is localized on human chromosome 17q23.


The specific activity of recombinant human ACE is measured by its ability to cleave hippuryl-HIS-LEU

Biochem/physiol Actions

Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. It is a membrane-bound enzyme, which is expressed in atherosclerotic lesions. It plays an important role in the development of various diseases like systemic lupus erythematosus, hypertension, chronic kidney disease and diabetic nephropathy.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1.0 micromole of hippuric acid from hippuryl-HIS-LEU per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 oC.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

Storage Class

11 - Combustible Solids




Not applicable


Not applicable

Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Documents related to the products that you have purchased in the past have been gathered in the Document Library for your convenience.

Visit the Document Library

Difficulty Finding Your Product Or Lot/Batch Number?

Product numbers are combined with Pack Sizes/Quantity when displayed on the website (example: T1503-25G). Please make sure you enter ONLY the product number in the Product Number field (example: T1503).


Product Number
Pack Size/Quantity

Additional examples:





enter as 1.000309185)

Having trouble? Feel free to contact Technical Service for assistance.

Lot and Batch Numbers can be found on a product's label following the words 'Lot' or 'Batch'.

Aldrich Products

  • For a lot number such as TO09019TO, enter it as 09019TO (without the first two letters 'TO').

  • For a lot number with a filling-code such as 05427ES-021, enter it as 05427ES (without the filling-code '-021').

  • For a lot number with a filling-code such as STBB0728K9, enter it as STBB0728 without the filling-code 'K9'.

Not Finding What You Are Looking For?

In some cases, a COA may not be available online. If your search was unable to find the COA you can request one.

Request COA

Angiotensin-Converting Enzyme in Smooth Muscle Cells Promotes Atherosclerosis
Chen X, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology (2016)
ACE Gene I/D Polymorphism and Obesity in 1,574 Patients with Type 2 Diabetes Mellitus.
Pan YH, et al.
Disease Markers (2016)
Local corticosterone production and angiotensin-I?converting enzyme shedding in a mouse model of intestinal inflammation
Salmenkari H, et al.
World Journal of Gastroenterology, 21(35), 10072-10072 (2015)
Genetic Polymorphism of Angiotensin-Converting Enzyme and Chronic Obstructive Pulmonary Disease Risk: An Updated Meta-Analysis.
Kang SW, et al.
BioMed Research International (2016)
Ander Vergara et al.
International journal of molecular sciences, 23(21) (2022-11-12)
Treatments with sodium-glucose 2 cotransporter inhibitors (SGLT2i) or endothelin receptor antagonists (ERA) have shown cardiorenal protective effects. The present study aimed to evaluate the cardiorenal beneficial effects of the combination of SGLT2i and ERA on top of renin-angiotensin system (RAS)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service