SAE0075

Sigma-Aldrich

Angiotensin Converting Enzyme, ACE, human

recombinant, ≥10 U/mg, expressed in HEK 293 cells

Synonym(s):
DCP1, ACE1, Kininase II, Peptidyldipeptide Hydrolase, CD143, Dipeptidyl carboxypeptidase I, ACE, Peptidyl-dipeptidase A, DCP
Enzyme Commission number:

recombinant

expressed in HEK 293 cells

Quality Level

assay

≥95% (SDS-PAGE)

form

lyophilized powder

specific activity

≥10 U/mg

mol wt

calculated mol wt 138 kDa (The protein migrates as a 180 kDa protein on SDS-PAGE due to glycosylation)

UniProt accession no.

shipped in

dry ice

storage temp.

−20°C

General description

The ACE gene codes for angiotensin-converting enzyme, which is one of the major enzymes in the RAS (renin angiotensin system). The ACE gene is localized on human chromosome 17q23.
Recombinant human Angiotensin Converting Enzyme (ACE) is expressed in human HEK 293 cells as a glycoprotein with a calculated molecular mass of 138 kDa (amino acids 30-1232). The DTT-reduced protein migrates as a 180 kDa polypeptide on SDS-PAGE due to glycosylation. This protein is manufactured in human cells, with no serum. The human cells expression system allows human-like glycosylation and folding, and often supports higher specific activity of the protein. The protein is produced with no artificial tags.

Specificity

The specific activity of recombinant human ACE is measured by its ability to cleave hippuryl-HIS-LEU

Biochem/physiol Actions

Angiotensin I converting enzyme (ACE) catalyzes the formation of angiotensin II from angiotensin I. It is a membrane-bound enzyme, which is expressed in atherosclerotic lesions. It plays an important role in the development of various diseases like systemic lupus erythematosus, hypertension, chronic kidney disease and diabetic nephropathy.

Unit Definition

One unit is defined as the amount of enzyme required to produce 1.0 micromole of hippuric acid from hippuryl-HIS-LEU per minute in 50 mM HEPES and 300 mM NaCl at pH 8.3 at 37 oC.

Physical form

Lyophilized from 0.22 μm filtered solution in PBS, pH7.4.

RIDADR

NONH for all modes of transport

WGK Germany

WGK 2

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Local corticosterone production and angiotensin-I?converting enzyme shedding in a mouse model of intestinal inflammation
Salmenkari H, et al.
World Journal of Gastroenterology, 21(35), 10072-10072 (2015)
Angiotensin-Converting Enzyme in Smooth Muscle Cells Promotes Atherosclerosis
Chen X, et al.
Arteriosclerosis, Thrombosis, and Vascular Biology (2016)
ACE Gene I/D Polymorphism and Obesity in 1,574 Patients with Type 2 Diabetes Mellitus.
Pan YH, et al.
Disease Markers (2016)
Genetic Polymorphism of Angiotensin-Converting Enzyme and Chronic Obstructive Pulmonary Disease Risk: An Updated Meta-Analysis.
Kang SW, et al.
BioMed Research International (2016)

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