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SAE0156

Sigma-Aldrich

Bone Alkaline Phosphatase from human bone osteosarcoma cells

lyophilized, ≥2000 u/mg protein

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Synonym(s):
ALPL, BAP, Bone ALP, Ostase

Quality Level

shipped in

wet ice

storage temp.

−20°C

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This Item
A7827MAK447RAP-RO
vibrant-m

MAK447

Alkaline Phosphatase Assay Kit

vibrant-m

RAP-RO

rAPid Alkaline Phosphatase

storage temp.

−20°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

100

Quality Level

100

General description

Mammalian alkaline phosphatases are a group of membrane-bound glycoprotein isoenzymes that are attached to the cell membrane by a hydrophobic glycosyl-phosphatidylinositol (GPI) anchor.
The bone alkaline phosphatase isozyme is expressed from the gene for tissue nonspecific alkaline phosphatase which is also expressed in liver and kidney. These three isoforms form the majority of alkaline phosphatases circulating in serum. All three forms share the same amino acid sequence but have different carbohydrate and lipid modifications that provide unique properties according to the source tissue.

Bone alkaline phosphatase is synthesized by osteoblasts, involved in the calcification of bone matrix, but its precise role in bone formation process is still unknown. It is a highly specific marker of the bone-forming activity of osteoblasts. High activity of bone alkaline phosphatase is observed in serum in several bone diseases, such as Paget′s disease, osteoporosis, ricket disease, bone metastatic carcinoma, and others. Physiological bone growth can also contribute to increased levels of bone alkaline phosphatase in serum.

This product is purified from human osteosarcoma Saos-2 cells, an established cell line with high basal alkaline phosphatase activity. Before purification, alkaline phosphatase is released from the cell membrane by enzymatic cleavage of the GPI anchor.

Unit Definition

One unit will hydrolyze 1.0 μmole of p-nitrophenyl phosphate to p-nitrophenol and inorganic phosphate per minute at pH 9.8 at 37 °C.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3


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