It is suggested to reconstitute the product in water or in 10% aqueous glycerol solution at a protein concentration of 1-2 mg/mL. Aliquot the protein solution and store the solution aliquots at –20 °C. Avoid freeze-thaw cycles.
SAE0172
Mpro, 3CL Protease from coronavirus SARS-COV-2
recombinant protein, lyophilized product
Synonym(s):
2019-nCoV, 3C Like proteinase, 3C-like main protease, 3CL Mpro, 3CL protease, 3CL proteinase, 3CLpro, COVID-19, COVID-2019, SARS-CoV-2, coronavirus
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| Size/SKU | Availability | Price |
|---|---|---|
200 μg | Estimated to ship onAugust 21, 2026fromMILWAUKEE | $546.00 |
About This Item
NACRES:
NA.54
UNSPSC Code:
12352204
Form:
lyophilized
Quality Segment
form
lyophilized
NCBI accession no.
shipped in
wet ice
storage temp.
−20°C
General description
Mpro, 3CL is a critical enzyme for the virus life cycle. It activity yield non-structural proteins that are crucial for genome replication and Coronavirus virion production: RNA-dependent RNA polymerase, a helicase, ribonucleases and 3CLpro itself, from two types of polyproteins (pp1a and pp1ab).The maturation of SARS-Cov2 virus (SARS coronavirus) depends on cleavage of the overlapping large polyproteins 1a and 1ab by two viral proteases:
SARS-CoV Mpro, 3CL exists as a homodimer and each protomer has an active site. The proteolytic cleavage of 1ab polyprotein by Mpro occurs at 11 sites: 7 sites within the 1a polyprotein, and 11 sites within the 1ab polyprotein. This results in maturation of 16 viral non-structural proteins.Mpro protease forms a functional homodimer. Both the N-terminus and the C- terminus of Mpro have been shown to be critical for dimer formation and for enzyme function.
The Mpro protease, 3CLpro is an ideal target for antiviral drug design due to its high conservation between different coronavirus strains and absence of functional analogs in the human proteome. Mpro protease from SARS-CoV1 and SARS-CoV2 are functionally identical.
- Mpro (main protease)
- PLpro (Papain-like protease)
SARS-CoV Mpro, 3CL exists as a homodimer and each protomer has an active site. The proteolytic cleavage of 1ab polyprotein by Mpro occurs at 11 sites: 7 sites within the 1a polyprotein, and 11 sites within the 1ab polyprotein. This results in maturation of 16 viral non-structural proteins.Mpro protease forms a functional homodimer. Both the N-terminus and the C- terminus of Mpro have been shown to be critical for dimer formation and for enzyme function.
The Mpro protease, 3CLpro is an ideal target for antiviral drug design due to its high conservation between different coronavirus strains and absence of functional analogs in the human proteome. Mpro protease from SARS-CoV1 and SARS-CoV2 are functionally identical.
Features and Benefits
Mpro, 3CL Protease (3C-like protease)is the main protease of Human Coronavirus SARS-CoV-2. Mpro 3CL Protease is a cysteine protease. Mpro protease cleaves proteins with sequences including LQ[S/A/G) c-terminal to the glutamine residue.
Physical form
The product is supplied lyophilized from 20 mM HEPES, 2.5% Trehalose, and 0.05% Tween 20
This product contains the complete sequence of Mpro protease (Accession: YP_009725301.1) without any additional tags. The product is supplied lyophilized from 20 mM HEPES, 2.5% Trehalose, and 0.05% Tween 20.
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This Item | |||
|---|---|---|---|
| form lyophilized | form liquid | form - | form aqueous solution |
| shipped in wet ice | shipped in dry ice | shipped in ambient | shipped in dry ice |
| storage temp. −20°C | storage temp. −70°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 100 | Quality Level 200 | Quality Level - |
| NCBI accession no. | NCBI accession no. - | NCBI accession no. - | NCBI accession no. - |
signalword
Danger
hcodes
pcodes
Hazard Classifications
Resp. Sens. 1
Storage Class
11 - Combustible Solids
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Dear Officer Please could you give me information on how to dissolve M-Pro Protease (SAE0172)? Which solvent to use? Also, what it is the optimal concentration for M-Pro to be stored? Thank you in advance
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