Skip to Content
MilliporeSigma

SAE0197

Sigma-Aldrich

Anti-HA Magnetic Beads

Magnetic agarose, suspension

Synonym(s):

HA beads

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

1 ML
$322.00

$322.00


Estimated to ship onJuly 18, 2025FromMILWAUKEE


Select a Size

Change View
1 ML
$322.00

About This Item

UNSPSC Code:
12352203
NACRES:
NA.56

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

$322.00


Estimated to ship onJuly 18, 2025FromMILWAUKEE


Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

conjugate

magnetic beads

Quality Level

form

suspension

shipped in

wet ice

storage temp.

2-8°C

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SAE0203M8823A2095
conjugate

magnetic beads

conjugate

magnetic beads

conjugate

magnetic beads

conjugate

agarose conjugate

form

suspension

form

suspension

form

suspension

form

PBS suspension

shipped in

wet ice

shipped in

ambient

shipped in

wet ice

shipped in

wet ice

storage temp.

2-8°C

storage temp.

2-8°C

storage temp.

−20°C

storage temp.

2-8°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Anti-HA Magnetic Beads are a conjugate of high-affinity mouse IgG1 monoclonal anti-HA antibody against HA tag (YPYDVPDYA) derived from human influenza the hemagglutinin (HA) protein. The high degree of charged amino acids in the HA-tag enhances its potential for strong antibody recognition. This product may be used for purification of HA-tagged proteins that are expressed in bacterial and mammalian cell lysates.

Application

These anti-HA magnetic agarose beads may be used in various immunological techniques, such as immunoprecipitation[1], immunoaffinity purification, ubiquitination assay.[2]

Features and Benefits

  • Antibody is conjugated to cyanogen bromide-activated magnetic agarose beads.
  • Protein-to-beads ratio ~ 4 mg/mL.
  • High specificity binding to HA-tagged proteins.

Other Notes

This product is for R&D use only. Not for drug, household, or other uses. Please consult the Safety Data Sheet for information regarding hazards and safe handling practices.

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 3


Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

Don't see the Right Version?

If you require a particular version, you can look up a specific certificate by the Lot or Batch number.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Questions

1–3 of 3 Questions  
  1. The beads are supplied as the suspension and the loading is described as 4mg of antibody for 1mg of beads. However the concentration of suspension is not specified. How many mg of beads are there in the 1ml of supplied suspension?

    1 answer
    1. This product does not have a specification that describes "mg of beads per one milliliter." The product is described as a "~ 4 mg/mL protein-to-beads ratio" meaning there is approximately 4 mg of antibody conjugated to 1 mL of beads. This is a description of the product and not a recommendation for use. The general guidance provided in the technical bulletin suggests using 20 µL of the anti-HA magnetic agarose beads, which have an estimated bead capacity of 50 µg. It is suggested to add a sufficient amount of cell lysate where 50 µg of the HA-tagged protein is expected to be present.

      Helpful?

  2. What is the average size of the beads?

    1 answer
    1. This product consists of a non-crosslinked magnetic agarose resin with a bead size of 20-75 µm.

      Helpful?

  3. Is the antibody covalently conjugated to the beads and will the HA-antibody comes off during elution or boiling?

    1 answer
    1. In SAE0197, the antibody is covalently conjugated to the beads, and the HA-antibody remains bonded to the beads during elution or boiling.

      Helpful?

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service