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SCJ4600016

Sigma-Aldrich

CF 488A maleimide

suitable for fluorescence, ≥90% (TLC)

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NACRES:
NA.41

Quality Level

assay

≥90% (TLC)

form

solid

mol wt

~1036 g/mol

solubility

DMF: soluble
DMSO: soluble
H2O: soluble

ε (extinction coefficient)

70000 at 490 nm

absorbance ratio

A280/490 nm 0.1

fluorescence

λex 488 nm (Flow cytometry laser line)
λex 488 nm (Microscopy laser line)
λex 490 nm; λem 515 nm

suitability

suitable for fluorescence

shipped in

wet ice

storage temp.

−20°C

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SCJ4600016

CF 488A maleimide

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CF 488A amine

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SCJ4600017

CF 488A aminooxy

CF™ 488A hydrazide suitable for fluorescence, ≥90% (HPLC)

SCJ4600015

CF 488A hydrazide

fluorescence

λex 488 nm (Flow cytometry laser line), λex 490 nm; λem 515 nm, λex 488 nm (Microscopy laser line)

fluorescence

λex 488 nm (Flow cytometry laser line), λex 488 nm (Microscopy laser line), λex 490 nm; λem 515 nm

fluorescence

λex 488 nm (Flow cytometry laser line), λex 488 nm (Microscopy laser line), λex 490 nm; λem 515 nm

fluorescence

λex 488 nm (Flow cytometry laser line), λex 488 nm (Microscopy laser line), λex 490 nm; λem 515 nm

form

solid

form

solid

form

solid

form

solid

solubility

DMF: soluble, H2O: soluble, DMSO: soluble

solubility

DMSO: soluble

solubility

DMF: soluble, DMSO: soluble

solubility

H2O: soluble

ε (extinction coefficient)

70000 at 490 nm

ε (extinction coefficient)

70000 at 490 nm

ε (extinction coefficient)

70000 at 490 nm

ε (extinction coefficient)

70000 at 490 nm

shipped in

wet ice

shipped in

-

shipped in

-

shipped in

-

General description

CF488A is a green fluorescent dye optimally excitable by the 488 nm argon laser line. Under common detection conditions, CF488A is at least as bright as Alexa Fluor 488. However, a major advantage of CF488A over Alexa Fluor 488 is that antibody conjugates prepared from the former are biologically more specific. Alexa Fluor 488 carries multiple negative charges, which can significantly change the isoelectric point of the proteins the dye labels and consequently alter the specificity of the protein conjugates. CF488A, on the other hand, is minimally charged. Thus, antibody conjugates prepared from the dye ensure biological detection with high signal-to-noise ratio. Another feature of CF488A is that the emission peak wavelength is about 10 nm shorter than that of Alexa Fluor 488 and 15 nm shorter than that of the traditional green dye FITC (or FAM). The shorter wavelength of CF488A offers the advantage of less fluorescence "spill-over" in the red channel in multi-color detection applications.

Application

CF 488A maleimide has been used to tag FtsN (cell division protein), a protein involved in bacterial cell division machinery.

Features and Benefits

1. Yields biologically more specific antibody conjugates and has less "spill-over" fluorescence in the red channel than Alexa Fluor488. 2. Extremely photostable. 3. Highly water-soluble and pH-insensitive.

Preparation Note

Protect from light

Reconstitution

Dissolve in DMSO

Legal Information

This product is distributed by Sigma-Aldrich Co. under the authorization of Biotium, Inc. This product is covered by one or more US patents and corresponding patent claims outside the US patents or pending applications owned or licensed by Biotium, Inc. including without limitation: 12/334,387; 12/607,915; 12/699,778; 12/850,578; 61/454,484. In consideration of the purchase price paid by the buyer, the buyer is hereby granted a limited, non-exclusive, non-transferable license to use only the purchased amount of the product solely for the buyer′s own internal research in a manner consistent with the accompanying product literature. Except as expressly granted herein, the sale of this product does not grant to or convey upon the buyer any license, expressly, by implication or estoppel, under any patent right or other intellectual property right of Biotium, Inc. Buyer shall not resell or transfer this product to any third party, or use the product for any commercial purposes, including without limitation, any diagnostic, therapeutic or prophylactic uses. This product is for research use only. Any other uses, including diagnostic uses, require a separate license from Biotium, Inc. For information on purchasing a license to use this product for purposes other than research, contact Biotium, Inc., 3159 Corporate Place, Hayward, CA 94545, Tel: (510) 265-1027. Fax: (510) 265-1352. Email: btinfo@biotium.com.
ALEXA FLUOR is a trademark of Life Technologies
CF is a trademark of Biotium, Inc.

Disclaimer

Unless otherwise stated in our catalog or other company documentation accompanying the product(s), our products are intended for research use only and are not to be used for any other purpose, which includes but is not limited to, unauthorized commercial uses, in vitro diagnostic uses, ex vivo or in vivo therapeutic uses or any type of consumption or application to humans or animals.

Storage Class

11 - Combustible Solids

wgk_germany

WGK 3

flash_point_f

Not applicable

flash_point_c

Not applicable


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