The fluorescence of a thrombin substrate, fluorogenic, typically increases upon cleavage by thrombin. These substrates are designed with a quenched fluorescence system, where the intact substrate exhibits low or no fluorescence. When thrombin cleaves the substrate at the specific recognition site, the fluorophore is separated from the quencher, resulting in an increased fluorescence signal.
SCP0252
Thrombin Substrate, fluorogenic
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5 MG
$317.00
About This Item
Empirical Formula (Hill Notation):
C31H42N7O7
Molecular Weight:
624.71
UNSPSC Code:
12352202
NACRES:
NA.32
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assay
≥95% (HPLC)
form
lyophilized
composition
Peptide Content, ≥80%
storage condition
protect from light
storage temp.
−20°C
1 of 4
This Item | 605211 | T7572 | T9326 |
|---|---|---|---|
| assay ≥95% (HPLC) | assay ≥98% (HPLC) | assay - | assay ≥95% (SDS-PAGE) |
| form lyophilized | form lyophilized | form lyophilized powder | form aqueous solution |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −70°C |
| storage condition protect from light | storage condition OK to freeze, desiccated | storage condition - | storage condition - |
| composition Peptide Content, ≥80% | composition - | composition - | composition - |
Amino Acid Sequence
BOC-Val-Pro-Arg-AFC
General description
Thrombin has procoagulant and anticoagulant properties. It also has chemotactic and mitogenic effects. Thrombin triggers the growth of mammalian cells, fibroblasts and macrophage- like tumor cell lines. It regulates brain development.[1] Thrombin plays an important role in hemostasis and thrombosis. It is associated with mast cell degranulation along with the release of histamine and enhances the production of IL-1 (interleukin-1) by activated macrophages. Thrombin stimulates vascular permeability and transendothelial cell migration by neutrophils.[2]
Application
Thrombin is a serine protease involved in the coagulation cascade wherein it converts fibrinogen into fibrin. Thrombin may be detected (assayed) using peptides that contain the VPR sequence such as the fluorogenic substrates Boc-VPR-AFC, Boc-Val-Pro-Arg-7-AMC and the chromogenic substrate Boc-Val-Pro-Arg-pNA. These substrates may also be useful to detect other serine proteases.
Storage Class
11 - Combustible Solids
wgk_germany
WGK 3
flash_point_f
Not applicable
flash_point_c
Not applicable
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Does the fluorescence of this product decrease when it is cleaved by thrombin?
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Is there any solubility information available for this product?
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As per the supplier the product is soluble at 3 mg per ml or greater.
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How do we use this product i.e., its dissolution medium and its fluorescent intensity wavelengths (excitation and emission)?
1 answer-
This material is used in research applications for testing mammalian cells as described in the general properties section of the product page. As per the supplier, the material can be solubilized in DMSO (> 3mg/ml) before use. The stock solution can be further diluted into buffer (e.g PBS). Stability of stock solutions is unrecorded. The Fluorophore is a Coumarin with an excitation wavelength at 365 nm and an emission wavelength at 440 nm (in ethanol(lit.)).
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