SEP100

Millipore

Seppro® Supermix Mouse

LC5

NACRES:
NA.32

Quality Level

column D × L

12.7 mm × 39.5 mm

capacity

loading(Flow Through volume from IgY 14 LC10 (SEP090))

storage temp.

2-8°C

General description

After the removal of highly abundant proteins (HAP) with the Seppro® mouse depletion column (SEP110 or SEP 090), the next level of protein abundance, the moderately abundant proteins (MAP) remains an obstacle in accessing the (low abundant proteins) LAP, where the majority of biologically interesting and clinically important biomarkers reside. Seppro Supermix Mouse can further partition complex plasma/serum samples. This product is generated by immunizing chickens with mouse plasma proteins from which the 7 HAP have been removed. The resulting antibodies are mainly against MAP and or immunoreactive proteins. Both the collected flow-through fraction and eluted fraction can be used for downstream analysis.

Application

Seppro® Supermix, LC5 (5 mL column) is used as avian polyclonal IgY-based immunoaffinity follow-on separation of mouse serum moderately abundant proteins (MAP) from mouse serum low abundant proteins (LAP) after specific mouse serum highly-abundant proteins (HAP) have been removed using Seppro® mouse depletion column SEP10.

Legal Information

Seppro is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • S4199Seppro® Dilution buffer

  • S4324Seppro® Stripping Buffer

  • S4449Seppro® Neutralization Buffer

  • CLS8163Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile

Pictograms

Exclamation mark

Signal Word

Warning

Hazard Statements

Precautionary Statements

RIDADR

NONH for all modes of transport

  1. How does the storage temperature relate to shipping conditions?

    The storage conditions that a Sigma-Aldrich catalog and label recommend for products are deliberately conservative. For many products, long-term storage at low temperatures will increase the time during which they are expected to remain in specification and therefore are labeled accordingly. Where short-term storage, shipping time frame, or exposure to conditions other than those recommended for long-term storage will not affect product quality, Sigma-Aldrich will ship at ambient temperature. The products sensitive to short-term exposure to conditions other than their recommended long-term storage are shipped on wet or dry ice. Ambient temperature shipping helps to control shipping costs for our customers. At any time, our customers can request wet- or dry-ice shipment, but the special handling is at customer expense if our product history indicates that the product is stable for regular shipment. See Shipping and Storage for more information.

  2. Which document(s) contains shelf-life or expiration date information for a given product?

    If available for a given product, the recommended re-test date or the expiration date can be found on the Certificate of Analysis.

  3. How do I get lot-specific information or a Certificate of Analysis?

    The lot specific COA document can be found by entering the lot number above under the "Documents" section.

  4. How do I find price and availability?

    There are several ways to find pricing and availability for our products. Once you log onto our website, you will find the price and availability displayed on the product detail page. You can contact any of our Customer Sales and Service offices to receive a quote.  USA customers:  1-800-325-3010 or view local office numbers.

  5. What is the Department of Transportation shipping information for this product?

    Transportation information can be found in Section 14 of the product's (M)SDS.To access the shipping information for this material, use the link on the product detail page for the product. 

  6. How many moderately abundant proteins (MAPs) does Product SEP100, Seppro® Supermix Mouse, deplete?

    As of this time, the exact number of depleted MAPs by SEP100 is not precisely defined.  This question is currently under investigation.

  7. My protein sample contains SDS.  Is this compatible with Seppro® Supermix Mouse?  

    No. We do not recommend using protein samples containing SDS with Product No. SEP100.  SDS is a detergent and would be expected to affect adversely the activity of the conjugated antibody in terms of depletion.

  8. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

  9. My question is not addressed here, how can I contact Technical Service for assistance?

    Ask a Scientist here.

Katrin König et al.
Clinical biochemistry, 46(7-8), 652-655 (2013-01-23)
Investigation of the practicability and performance of a magnetic micro-particle based method for protein depletion of serum samples, preceding the quantitative analysis of small molecules by LC-MS/MS. A commercially available kit including a protein denaturation reagent and functionalized magnetic particles...
Majlinda Kullolli et al.
Journal of chromatography. B, Analytical technologies in the biomedical and life sciences, 939, 10-16 (2013-10-05)
Human plasma is a commonly used diagnostic fluid in clinical chemistry. In-depth plasma proteomic analysis is performed to search for disease biomarkers, however the large dynamic range of protein abundance in plasma presents a substantial analytical challenge. Removal of abundant...

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