SEP130

Millipore

Seppro® Rat

Spin Columns

NACRES:
NA.32

Quality Level

capacity

1.2 mg total protein loading(26 μL of rat plasma, average rat protein concentration 45 mg/mL)

storage temp.

2-8°C

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General description

Immunoaffinity partitioning of highly-abundant proteins (HAP) has proven to be one of the most effective approaches for overcoming the wide dynamic range of plasma protein concentration, as well as enabling the detection of low-abundant proteins (LAP). Avian polyclonal IgY (Immunoglobulin Yolk) antibodies have unique and advantageous features that allow highly-specific partitioning of protein mixtures. Sigma offers a novel Seppro® IgY-R7 column system, which takes you deeper into the rat plasma/serum proteome.

Proteins Partitioned:

Rat serum albumin
IgG
Fibrinogen
Transferrin
IgM
Haptoglobin
alpha1-Antitrypsin

Application

Seppro® Rat, spin columns (15-20μL capacity) is designed for use as a plasma/serum protein removal tool base upon avian antibody (IgY)-antigen immune affinity removal of seven highly abundant proteins (HAP) from rat plasma protein mixtures via liquid chromatography. Seppro® Rat, spin column is used to support the analysis of low abundance rat serum/plasma proteins.

Legal Information

Seppro is a registered trademark of Sigma-Aldrich Co. LLC

Kit Components Also Available Separately

Product No.
Description
SDS

  • S4199Seppro® Dilution buffer

  • S4324Seppro® Stripping Buffer

  • S4449Seppro® Neutralization Buffer

  • CLS8163Corning® Costar® Spin-X® centrifuge tube filters, cellulose acetate membrane, pore size 0.45 μm, non-sterile

  • S4574Spin Column for Seppro®

Pictograms

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Warning

Hazard Statements

Precautionary Statements

RIDADR

NONH for all modes of transport

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