MilliporeSigma
All Photos(1)

SHC002

Sigma-Aldrich

MISSION® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA

Targets no known mammalian genes

Synonym(s):
MISSION Control Vectors
MDL number:
NACRES:
NA.51

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Application

MISSION® pLKO.1-puro non-mammalian shRNA control plasmid DNA has been used:
  • to transduce RLE-6TN cells
  • for the validation of Oct4-green fluorescent protein (GFP) mouse embryonic fibroblasts (MEFs)
  • as a negative control for RNA knockdown

MISSION®pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA has been used:
  • for the knockdown of transmembrane prostate androgen-induced RNA (TMEPAI)
  • in the infection mixture for lentivirus preparation
  • for generating the puromycin-resistant ST14A (rat striatal neuronal cell line) cell populations
  • to induce Ago2 (argonaute-2) down-regulation

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 1

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable

Personal Protective Equipment

dust mask type N95 (US), Eyeshields, Gloves

Certificate of Analysis

Enter Lot Number to search for Certificate of Analysis (COA).

Certificate of Origin

Enter Lot Number to search for Certificate of Origin (COO).

TMEPAI/PMEPA 1 enhances tumorigenic activities in lung cancer cells
Vo Nguyen TT, et al.
Cancer Science, 105(3), 334-341 (2014)
Identification of a gene expression signature associated with the metastasis suppressor function of NME1: prognostic value in human melanoma
Leonard MK, et al.
Laboratory Investigation; a Journal of Technical Methods and Pathology, 98(3), 327-327 (2018)
SFPQ?NONO and XLF function separately and together to promote DNA double-strand break repair via canonical nonhomologous end joining.
Jaafar l
Nucleic Acids Research, 45(4), 1848-1859 (2017)
Mutant huntingtin alters cell fate in response to microtubule depolymerization via the GEF-H1-RhoA-ERK pathway
Varma H, et al.
The Journal of Biological Chemistry, 285(48), 37445-37457 (2010)
TRIM28 is an epigenetic barrier to induced pluripotent stem cell reprogramming
Miles DC, et al.
Stem Cells, 35(1), 147-157 (2017)

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service