SHC002

MISSION^® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA

Targets no known mammalian genes

• Synonym(s): MISSION^® Control Vectors
• MDL number: MFCD07785395
• NACRES: NA.51

Properties

• Quality Level: 200
• product line: MISSION^®
• concentration: 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
• shipped in: dry ice
• storage temp.: −20°C

Description

Application

MISSION^® pLKO.1-puro non-mammalian shRNA control plasmid DNA has been used:

• to transduce RLE-6TN cells
• for the validation of Oct4-green fluorescent protein (GFP) mouse embryonic fibroblasts (MEFs)
• as a negative control for RNA knockdown

MISSION^®pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA has been used:

• for the knockdown of transmembrane prostate androgen-induced RNA (TMEPAI)
• in the infection mixture for lentivirus preparation
• for generating the puromycin-resistant ST14A (rat striatal neuronal cell line) cell populations
• to induce Ago2 (argonaute-2) down-regulation

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Sigma-Aldrich Co. LLC

Safety Information

• Storage Class Code: 12 - Non Combustible Liquids
• WGK: WGK 1
• Flash Point(F): Not applicable
• Flash Point(C): Not applicable
• Personal Protective Equipment: dust mask type N95 (US), Eyeshields, Gloves