SHC004

MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Plasmid DNA

Name: MISSION<SUP>®</SUP> pLKO.1-puro TurboGFP<SUP>™</SUP> shRNA Control Plasmid DNA
Brand: SIGMA
Price: 441 USD

shRNA sequence targeting tGFP

Synonym(s):

MISSION^® Control Vectors

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About This Item

UNSPSC Code:

41106609

NACRES:

NA.51

MDL number:

MFCD07785395

Key Documents

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Product Name

MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

product line

MISSION^®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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Show Differences

1 of 4

This Item	SHC204	SHC003	SHC005
Sigma-Aldrich SHC004 MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Plasmid DNA	Sigma-Aldrich SHC204 MISSION^® TRC2 pLKO.5-puro TurboGFP^™ shRNA Control Plasmid DNA	Sigma-Aldrich SHC003 MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Plasmid DNA	Sigma-Aldrich SHC005 MISSION^® pLKO.1-puro eGFP shRNA Control Plasmid DNA
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 100	Quality Level 200	Quality Level 200

Application

MISSION^® pLKO.1-puro TurboGFP^™ shRNA control plasmid DNA has been used as lentiviral transduction and RNA interference assay.[1][2][3]

To see more application data, protocols, vector maps visit sigma.com/shrna.

General description

The MISSION TurboGFP shRNA Control Vector is a 7,087 base pair lentivirus plasmid vector that contains an shRNA sequence targeting TurboGFP.[4] The TurboGFP shRNA Control Vector is useful as a positive knockdown control in experiments using the MISSION TurboGFP positive control vector or in cell lines expressing TurboGFP. TurboGFP is an improved variant of the green fluorescent protein copGFP cloned from copepoda Pontellina plumata.
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids.[5][6] The TurboGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

TurboGFP is a trademark of Evrogen Co.

related product

Product No.

Description

Pricing

SHC002

MISSION^® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC001V

MISSION^® pLKO.1-puro Empty Vector Control Transduction Particles, Contains no shRNA insert

SHC003

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC002V

MISSION^® pLKO.1-puro Non-Mammalian shRNA Control Transduction Particles, Targets no known mammalian genes

SHC003V

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Transduction Particles, Green fluorescent protein marker to monitor transduction efficiency

SHC004V

MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Transduction Particles, shRNA sequence targeting tGFP

SHM01

MISSION^® ExpressMag^® Super Magnetic Kit, Increases transduction efficiency

SHM02

MISSION^® ExpressMag^® 96-Well Magnetic Kit, Increases transduction efficiency

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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Blocking Hedgehog release from pancreatic cancer cells increases paracrine signaling potency.

Damhofer H, et al.

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The MEK2-binding tumor suppressor hDlg is recruited by E-cadherin to the midbody ring

Gaudet S, et al.

BMC Cell Biology, 12(1), 55-55 (2011)

Loss of mutL homolog-1 (MLH1) expression promotes acquisition of oncogenic and inhibitor-resistant point mutations in tyrosine kinases.

Lorraine Springuel et al.

Cellular and molecular life sciences : CMLS, 73(24), 4739-4748 (2016-07-21)

Genomic instability drives cancer progression by promoting genetic abnormalities that allow for the multi-step clonal selection of cells with growth advantages. We previously reported that the IL-9-dependent TS1 cell line sequentially acquired activating substitutions in JAK1 and JAK3 upon successive

CUL1 Knockdown Attenuates the Adhesion, Invasion, and Migration of Triple-Negative Breast Cancer Cells via Inhibition of Epithelial-Mesenchymal Transition.

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Pathology oncology research : POR, 26(2), 1153-1163 (2019-06-09)

Cullin-1 (CUL1) is an important factor for tumor growth and a potential therapeutic target for breast cancer therapy, but the molecular mechanism in triple-negative breast cancer (TNBC) is unknown. In the present study, CUL1 shRNA was transfected into BT549 and

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MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA