SHC005

MISSION^® pLKO.1-puro eGFP shRNA Control Plasmid DNA

Name: MISSION<SUP>®</SUP> pLKO.1-puro eGFP shRNA Control Plasmid DNA
Brand: SIGMA
Price: 441 USD

shRNA sequence targeting eGFP

Synonym(s):

MISSION^® Control Vectors

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SHC005

$441.00

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About This Item

UNSPSC Code:

41106609

NACRES:

NA.51

MDL number:

MFCD07785395

Concentration:

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Key Documents

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product line

MISSION^®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Quality Level

200

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Show Differences

1 of 4

This Item	SHC004	SHC007	SHC003
Sigma-Aldrich SHC005 MISSION^® pLKO.1-puro eGFP shRNA Control Plasmid DNA	Sigma-Aldrich SHC004 MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Plasmid DNA	Sigma-Aldrich SHC007 MISSION^® pLKO.1-puro Luciferase shRNA Control Plasmid DNA	Sigma-Aldrich SHC003 MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Plasmid DNA
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −20°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

The MISSION^® eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761).[1] The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP.[2][3]
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids[4], MISSION^® Lentiviral Packaging Mix (Prod. No. SHP001). The eGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

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MISSION^® pLKO.1-puro eGFP shRNA Control Plasmid DNA