Skip to Content
MilliporeSigma

SHC005

Sigma-Aldrich

MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA

shRNA sequence targeting eGFP

Synonym(s):

MISSION® Control Vectors

Slide 1 of 1
Sign Into View Organizational & Contract Pricing

Select a Size

1 PKG
$441.00

$441.00

Check Cart for Availability
Request a Bulk OrderRequest more information

About This Item

MDL number:
MFCD07785395
UNSPSC Code:
41106609
NACRES:
NA.51

Key Documents

View All Documentation

Skip To

Technical Service
Need help? Our team of experienced scientists is here for you.
Let Us Assist

Quality Level

200

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

Looking for similar products? Visit Product Comparison Guide

Related Categories

Compare Similar Items

View Full Comparison

Show Differences

1 of 4

This Item
SHC004SHC007SHC003
MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA shRNA sequence targeting eGFP

Sigma-Aldrich

SHC005

MISSION® pLKO.1-puro eGFP shRNA Control Plasmid DNA

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA shRNA sequence targeting tGFP

Sigma-Aldrich

SHC004

MISSION® pLKO.1-puro TurboGFP™ shRNA Control Plasmid DNA

MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA shRNA sequence targeting luciferase

Sigma-Aldrich

SHC007

MISSION® pLKO.1-puro Luciferase shRNA Control Plasmid DNA

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA Green fluorescent protein marker to monitor transduction efficiency

Sigma-Aldrich

SHC003

MISSION® pLKO.1-puro-CMV-TurboGFP™ Positive Control Plasmid DNA

product line

MISSION®

product line

MISSION®

product line

MISSION®

product line

MISSION®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

The MISSION® eGFP shRNA Control Vector is a 7,091 base pair lentivirus plasmid vector that contains an shRNA sequence targeting eGFP (GenBank Accession No. pEGFP U55761).[1] The eGFP shRNA Control Vector is useful as a positive knockdown control in experiments using cell lines expressing eGFP.[2][3]
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids[4], MISSION® Lentiviral Packaging Mix (Prod. No. SHP001). The eGFP shRNA Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class

12 - Non Combustible Liquids

wgk_germany

WGK 1

flash_point_f

Not applicable

flash_point_c

Not applicable

Choose from one of the most recent versions:

Certificates of Analysis (COA)

Lot/Batch Number

It looks like we've run into a problem, but you can still download Certificates of Analysis from our Documents section.

If you need assistance, please contact Customer Support

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Huizhi Wang et al.
Oncogenesis, 9(8), 76-76 (2020-08-28)
Methyl-CpG-binding protein 2 (MeCP2) has been characterized as an oncogene in several types of cancer. However, its precise role in pancreatic ductal adenocarcinoma (PDAC) remains unclear. Hence, this study aimed to evaluate the potential role of MeCP2 in pancreatic cancer
Myoferlin regulates cellular lipid metabolism and promotes metastases in triple-negative breast cancer.
Blomme A
Oncogene, 36(15), 2116-2130 (2017)
Jun Won Park et al.
Biochemical and biophysical research communications, 498(3), 566-572 (2018-03-11)
TXNIP is a potent tumor suppressor with reduced expression in various types of human cancer. The prognostic and predictive power of TXNIP has been recognized in human breast cancer. The aim of this study is to investigate the clinical relevance
Alexandra Veloso et al.
Cell death & disease, 10(7), 512-512 (2019-07-06)
Muscle formation is controlled by a number of key myogenic transcriptional regulators that govern stage-specific gene expression programs and act as terminal effectors of intracellular signaling pathways. To date, the role of phosphatases in the signaling cascades instructing muscle development
Arnaud Blomme et al.
Oncotarget, 7(50), 83669-83683 (2016-11-16)
Exosomes are communication mediators participating in the intercellular exchange of proteins, metabolites and nucleic acids. Recent studies have demonstrated that exosomes are characterized by a unique proteomic composition that is distinct from the cellular one. The mechanisms responsible for determining
View All Related Papers

Questions

Reviews

No rating value

Active Filters

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service