Please see the link below to the sequence for the SHC012 - CMV-TagRFP™ Control.
https://www.sigmaaldrich.com/deepweb/assets/sigmaaldrich/marketing/global/documents/vectors/shc012-plko1-puro-cmv-tagrfp.txt
SHC012
MISSION® pLKO.1-puro-CMV-TagRFP™ Positive Control Plasmid DNA
Contains a gene encoding TagRFP
Synonym(s):
MISSION® Control Vectors
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1 PKG
$696.00
About This Item
Concentration:
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
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This Item | SHC014 | SHC005 | SHC003 |
|---|---|---|---|
| product line MISSION® | product line MISSION® | product line MISSION® | product line MISSION® |
| concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) | concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA) |
| shipped in dry ice | shipped in dry ice | shipped in dry ice | shipped in dry ice |
| storage temp. −20°C | storage temp. −20°C | storage temp. −20°C | storage temp. −20°C |
| Quality Level 200 | Quality Level 200 | Quality Level 200 | Quality Level 200 |
General description
Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, we recommend producing self-inactivating replication incompetent viral particles in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The CMV-TagRFP Control Vector is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
To see more application data, protocols, vector maps visit sigma.com/shrna.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
The MISSION TagRFP Control Vector is an 8594 base pair lentivirus plasmid vector that contains a gene encoding TagRFP, under the control of the CMV promoter. The CMV-TagRFP Control Vector is useful as a positive control in experiments using the MISSION shRNA library clones.
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany
TagRFP is a trademark of Evrogen Co.
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When shRNA is delivered using lentiviral vectors, the sequence encoding the shRNA is integrated into the genome and the knockdown effect is passed on to daughter cells, continuing gene silencing.
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