SHC016

MISSION^® pLKO.1-puro Non-Target shRNA Control Plasmid DNA

Name: MISSION<SUP>®</SUP> pLKO.1-puro Non-Target shRNA Control Plasmid DNA
Brand: SIGMA
Price: 431 USD

Targets no known genes from any species

Synonym(s):

MISSION^® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control

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About This Item

UNSPSC Code:

41106609

NACRES:

NA.51

MDL number:

MFCD07785395

Concentration:

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

Key Documents

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product line

MISSION^®

concentration

500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)

shipped in

dry ice

storage temp.

−20°C

Quality Level

200

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This Item	SHC216	SHC001	SHC016H
Sigma-Aldrich SHC016 MISSION^® pLKO.1-puro Non-Target shRNA Control Plasmid DNA	Sigma-Aldrich SHC216 MISSION^® TRC2 pLKO.5-puro Non-Target shRNA Control Plasmid DNA	Sigma-Aldrich SHC001 MISSION^® pLKO.1-puro Empty Vector Control Plasmid DNA	Sigma-Aldrich SHC016H MISSION^® pLKO.1-puro Non-Target shRNA Control Transduction Particles, High Titer
concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration 500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)	concentration ≥1x10⁹ VP/ml (via p24 assay)
product line MISSION^®	product line MISSION^®	product line MISSION^®	product line MISSION^®
shipped in dry ice	shipped in dry ice	shipped in dry ice	shipped in dry ice
storage temp. −20°C	storage temp. −20°C	storage temp. −20°C	storage temp. −70°C
Quality Level 200	Quality Level 200	Quality Level 200	Quality Level 200

General description

The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.

Application

MISSION^® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:

in tumor cells for multicolour imaging[1]
in human adult low calcium temperature keratinocytes[2]
in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)[3]
to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation[4]

To see more application data, protocols, vector maps visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

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SHC002

MISSION^® pLKO.1-puro Non-Mammalian shRNA Control Plasmid DNA, Targets no known mammalian genes

SHC003

MISSION^® pLKO.1-puro-CMV-TurboGFP^™ Positive Control Plasmid DNA, Green fluorescent protein marker to monitor transduction efficiency

SHC004

MISSION^® pLKO.1-puro TurboGFP^™ shRNA Control Plasmid DNA, shRNA sequence targeting tGFP

SHC005

MISSION^® pLKO.1-puro eGFP shRNA Control Plasmid DNA, shRNA sequence targeting eGFP

SHC007

MISSION^® pLKO.1-puro Luciferase shRNA Control Plasmid DNA, shRNA sequence targeting luciferase

SHC012

MISSION^® pLKO.1-puro-CMV-TagRFP^™ Positive Control Plasmid DNA, Contains a gene encoding TagRFP

SHC014

MISSION^® pLKO.1-puro-UbC-TurboGFP^™ Positive Control Plasmid DNA, Contains a gene encoding TurboGFP, under the control of the UbC promoter

Storage Class

10 - Combustible liquids

wgk

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable

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Zac1 regulates astroglial differentiation of neural stem cells through Socs3

Schmidt EU, et al.

Stem Cells, 31(8), 1621-1632 (2013)

Peroxiredoxin 2 nuclear levels are regulated by circadian clock synchronization in human keratinocytes

Avitabile D, et al.

The International Journal of Biochemistry & Cell Biology, 53(7580), 24-34 (2014)

Tumor microtubes convey resistance to surgical lesions and chemotherapy in gliomas.

Sophie Weil et al.

Neuro-oncology, 19(10), 1316-1326 (2017-04-19)

Primary and adaptive resistance against chemo- and radiotherapy and local recurrence after surgery limit the benefits from these standard treatments in glioma patients. Recently we found that glioma cells can extend ultra-long membrane protrusions, "tumor microtubes" (TMs), for brain invasion

Deletion of inositol hexakisphosphate kinase 1 (IP6K1) reduces cell migration and invasion, conferring protection from aerodigestive tract carcinoma in mice

Jadav RS, et al.

Cellular Signalling, 28(8), 1124-1136 (2016)

Brain tumour cells interconnect to a functional and resistant network

Osswald M, et al.

Nature, 528(7580), 93-93 (2015)

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MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA