- Genetic Screening
- SHC016
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SHC016
MISSION® pLKO.1-puro Non-Target shRNA Control Plasmid DNA
Targets no known genes from any species
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Synonym(s):
MISSION® Control Vectors, negative control, negative shRNA control, non-target control, non-target shRNA, non-target shRNA control, shRNA control
MDL number:
NACRES:
NA.51
Recommended Products
Quality Level
product line
MISSION®
concentration
500 ng/μL in TE buffer; DNA (10μg of plasmid DNA)
shipped in
dry ice
storage temp.
−20°C
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General description
The MISSION pLKO.1-puro Non-Target shRNA Control Plasmid DNA is a lentivirus plasmid vector. The vector contains an shRNA insert that does not target any known genes from any species, making it useful as a negative control in experiments using the MISSION shRNA library clones. This allows one to examine the effect of transfection of a short-hairpin on gene expression and interpret the knockdown effect seen with shRNA clones. Ampicillin and puromycin antibiotic resistance genes provide selection in bacterial or mammalian cells respectively. In addition, self-inactivating replication incompetent viral particles can be produced in packaging cells (HEK293T) by co-transfection with compatible packaging plasmids. The Non-Target shRNA Control Plasmid DNA is provided as 10 μg of plasmid DNA in Tris-EDTA (TE) buffer at a concentration of 500 ng/μl.
Application
MISSION® pLKO.1-puro non-target shRNA control plasmid DNA has been used as a control during transduction:
- in tumor cells for multicolour imaging
- in human adult low calcium temperature keratinocytes
- in mouse embryonic fibroblasts, to study the biological functions of IP6K1 (inositol hexakisphosphate kinase)
- to study the function of Zac1 (zinc finger protein regulating apoptosis and cell cycle arrest) expression in astroglial differentiation
Packaging
1 ea
Legal Information
Use of this product is subject to one or more license agreements. For details, please see http://sigmaaldrich.com/missionlicense.
MISSION is a registered trademark of Sigma-Aldrich Co. LLC
Storage Class Code
12 - Non Combustible Liquids
WGK
WGK 2
Flash Point(F)
Not applicable
Flash Point(C)
Not applicable
Certificate of Analysis
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Certificate of Origin
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Product Information Sheet
Vector
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