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SHCLNV

Sigma-Aldrich

MISSION® shRNA

Lentiviral Particles

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technique(s)

capture ELISA: 106 VP/mL using p24

shipped in

dry ice

storage temp.

−70°C

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This Item
CSTVRSSHCLNGSHCLND
Sigma-Aldrich

Sigma-Aldrich

SHCLNV

MISSION® shRNA

Sigma-Aldrich

Sigma-Aldrich

CSTVRS

MISSION® shRNA

Sigma-Aldrich

Sigma-Aldrich

SHCLNG

MISSION® shRNA

Sigma-Aldrich

Sigma-Aldrich

SHCLND

MISSION® shRNA

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

shipped in

dry ice

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−70°C

storage temp.

−20°C

Quality Level

200

Quality Level

200

Quality Level

200

Quality Level

200

General description

Our comprehensive shRNA product offering consists of over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes. The library is available in: gene family sets: gene collections related to specific functional classes such as kinases and ion channels; gene taget sets, sets of shRNA clones that target your favorite gene; and individual shRNA clones. Our shRNA and lentiviral manufacturing platforms utilize dedicated laboratories featuring state-of-the-art robotics, liquid handling and laboratory information management systems (LIMS) to provide superior quality and service for your RNAi research needs.
Lentiviral particles are ideal for transducing a wide range of cell lines. VSV-G Psuedotyping of the MISSION® lentiviral particles allows for entry into a wide range of cells. In addition, lentiviral particles stably integrate the shRNA into the genomes of both dividing and non-dividing cell lines. The MISSION bioproduction team regularly produces high quality lentivirus, so that our customers can get straight to their RNAi experiment without wasting precious time on tedious lentiviral production. Our minimum titer is 106 VP/mL. Unlike adenoviral infections that require vast excesses, lentiviral transduction is efficient enought allow for MOI as low as 1.

Application

MISSION® shRNA has been used:
  • in transfections and transductions
  • in the establishment of stable cell lines expressing shRNA
  • in the generation of alpha-actinin-4 (ACTN4) knockdown cell line
  • to transduce raw 264.7 cells

Other Notes

To see protocols and application data please visit sigma.com/shrna.

Legal Information

Use of this product is subject to one or more license agreements.
MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

Storage Class Code

12 - Non Combustible Liquids

WGK

WGK 3

Flash Point(F)

Not applicable

Flash Point(C)

Not applicable


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Barbara D Boyan et al.
Scientific reports, 8(1), 8588-8588 (2018-06-07)
Successful osseointegration of an endosseous implant involves migration and differentiation of mesenchymal stem cells (MSCs) on the implant surface. Micro-structured, hydrophilic titanium surfaces direct MSCs to undergo osteoblastic differentiation in vitro, in the absence of media additives commonly used in
Vera Mugoni et al.
Cell research, 29(6), 446-459 (2019-04-27)
Although targeted therapies have proven effective and even curative in human leukaemia, resistance often ensues. IDH enzymes are mutated in ~20% of human AML, with targeted therapies under clinical evaluation. We here characterize leukaemia evolution from mutant IDH2 (mIDH2)-dependence to
alpha-Actinin-4 confers radioresistance coupled invasiveness in breast cancer cells through AKT pathway
Desai S, et al.
Biochimica et Biophysica Acta - Molecular Cell Research, 1865(1), 196-208 (2018)
Bile acids reduce endocytosis of high-density lipoprotein (HDL) in HepG2 cells.
Rohrl C, Eigner K, Fruhwurth S, et al.
PLoS ONE, 9(7), e102026-e102026 (2014)
Sejal Desai et al.
Biochimica et biophysica acta, 1865(1), 196-208 (2017-10-23)
Acquired radioresistance accompanied with increased metastatic potential is a major hurdle in effective radiotherapy of breast cancers. However, the nature of their inter-dependence and the underlying mechanism remains largely intangible. By employing radioresistant (RR) cell lines, we herein demonstrate that

Articles

MISSION shRNA reduce the expression of specific target genes by targeting the specific mRNA therefore reducing the corresponding protein expression.

Our lentiviral vector systems are developed with enhanced safety features. Numerous precautions are in place in the design of our lentiviruses to prevent replication. Good handling practices are a must.

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Protocols

You are not alone designing successful CRISPR, RNAi, and ORF experiments. We were the first company to commercially offer lentivirus versions of targeted genome modification technologies and has the expertise and commitment to support new generations of scientists.

FACS (Fluorescence-Activated Cell Sorting) provides a method for sorting a mixed population of cells into two or more groups, one cell at a time, based on the specific light scattering and fluorescence of each cell. This method provides fast, objective, and quantitative recording of fluorescent signals from individual cells.

Related Content

Find shRNAs for Individual Genes. Over 150,000 pre-cloned shRNA constructs targeting more than 15,000 human and 15,000 mouse genes.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service