Skip to Content
MilliporeSigma
All Photos(1)

Documents

SHP002

Sigma-Aldrich

CRISPR & MISSION® Lentiviral Packaging Mix

Synonym(s):

Lentiviral Packaging Mix

Sign Into View Organizational & Contract Pricing


About This Item

UNSPSC Code:
41106609
NACRES:
NA.51

concentration

100 ng/μL (in Tris-EDTA)

Quality Level

application(s)

CRISPR

shipped in

dry ice

storage temp.

−20°C

General description

The CRISPR & MISSION Lentiviral Packaging Mix is an optimized formulation of two plasmids expressing the key HIV packaging genes and a heterologous viral envelope gene.

Lentiviral particles are generated from three main components:
  • The packaging vector, which contains the minimal set of lentiviral genes required to generate the virion structural proteins and packaging functions.
  • The vesicular stomatitis virus G-protein envelope vector, which provides the heterologous envelope for pseudotyping and allows these lentiviral particles to efficiently deliver the transfer sequence of interest to a wide variety of cell types, including primary and non-dividing cells
  • The lentiviral transfer vector, which contains the sequence of interest as well as the cis acting sequences necessary for packaging.

The Lentiviral Packaging Mix contains the first two components; it is designed to be co-transfected along with a compatible lentiviral transfer vector in order to create high-titer pseudo-typed lentiviral particles used for downstream transduction applications. This product is recommended for packaging Lenti CRISPR, Lenti ORF, and Lenti shRNA vectors.

Application

Functional Genomics/Screening/Target Validation

Legal Information

MISSION is a registered trademark of Merck KGaA, Darmstadt, Germany

wgk_germany

WGK 2

flash_point_f

Not applicable

flash_point_c

Not applicable


Certificates of Analysis (COA)

Search for Certificates of Analysis (COA) by entering the products Lot/Batch Number. Lot and Batch Numbers can be found on a product’s label following the words ‘Lot’ or ‘Batch’.

Already Own This Product?

Find documentation for the products that you have recently purchased in the Document Library.

Visit the Document Library

Articles

Lentiviral vector systems prioritize safety features, with design precautions preventing replication. Good handling practices are essential for use.

Get tips for handling lentiviruses, optimizing experiment setup, titering lentivirus particles, and selecting helpful products for transduction.

Protocols

Lentivirus versions of genome modification technologies support successful CRISPR, RNAi, and ORF experiments.

FACS sorts cells based on light scattering and fluorescence for objective cell analysis.

Our team of scientists has experience in all areas of research including Life Science, Material Science, Chemical Synthesis, Chromatography, Analytical and many others.

Contact Technical Service