MISSION^® siRNA Product Offerings

PREDESIGNED & Custom esiRNA

Biologically-prepared, esiRNA are comprised of a heterogeneous pool of siRNA (natural RNA, no modifications) that all target the same mRNA sequence.

• Format: Individual esiRNA, panels, and libraries available. Individual: 20 or 50 µg lyophilized in tubes. Panels & libraries: 1, 2.5, or 5 µg in solution in plates
• Purification: Q-Sepharose separation, isopropanol precipitation & ethanol washing
• Sequence Form: Pool of hundreds of siRNA with an average duplex length of 21 bp
• Quality Control: PCR product of cDNA clone is analyzed by gel electrophoresis & DNA sequencing; digestion reaction is analyzed by gel electrophoresis
• Learn more

Predesigned siRNA

Extensive collection of human, mouse and rat siRNA. Multiple sequences are available for every gene, with rankings to indicate performance.

• Scale: 10, 25, 50, and 250 nmol
• Purification: Desalt or HPLC
• Sequence Length: 21 bases
• Modifications: Not available for this item
• Quality Control: Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.
• Format: Supplied dry

Custom siRNA

Custom siRNA sequences with a wide range of modifications, purifications and quantities to tailor your unique sequence.

• Scale: 10, 25, 50, and 250 nmol
• Purification: Desalt or HPLC
• Sequence Lengths: 19 - 27 bases
• Modifications: Comprehensive offering of modifications and dyes (2'-O-Methyl, Amine, Cyanine dyes, 6-FAM, Biotin, Phosphate, Thiol, Abasic, Phosphorothioate linkages, 2'-Fluoro).
• Quality Control: Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.
• For additional purifications, sequence length or modifications,
  email sirnarequest@sial.com
• Custom siRNA sequence design available for a variety of species,
  Learn more

Fast siRNA

Expedite your siRNA order! Orders are prioritized in the production process with a turn-around-time of only 3 days.

• FAST siRNA is delivered in solution at concentrations of 50 µM (2 OD) or 100 µM (5 OD).
• US orders must be received by 11 AM CST. European orders must be received by 11 AM CET.
• Easy to use Fast siRNA ordering tool.

iScale Oligos™ - siRNA

siRNA provided in larger quantities for in vivo, high throughput or diagnostic projects.

• Amount: 1 – 10 mg, 50 mg – 1 g
• Purification: Desalt, RPC (cartridge purified), HPLC and In vivo quality
• Sequence Length: 19 - 45 bases
• Comprehensive offering of modifications including dyes, amines, biotin, linkers, etc.
• Quality Control: Individual strands are analyzed by mass spectrometry. Depending on manufacturing site, PAGE may be used to assess siRNA duplexes. siRNA quantity is systematically validated by UV absorbance at 260 nm.
• In vivo option includes HPLC-purification to >/= 90%, desalt, filtration and endotoxin testing.
• To inquire for availability of additional quantities, modifications or purifications, email sirnarequest@sial.com

siRNA Controls

Universal Negative Controls

• Two distinct siRNA control sequences designed for use in Human, Mouse, or Rat cells.
• Validated with Agilent 40K human gene arrays to ensure no significant nonspecific gene interactions.
• Functionally validated to show no immune response via qRT PCR.

Positive Control siRNA.

• Validated human and rat positive controls to simplify transfection optimization.

Custom miRNA

Custom miRNA sequences with a wide range of modifications, purifications, and quantities to tailor your unique sequence.

• Amount: 2 OD (10 nmol), 5 OD (25 nmol), 10 OD (50 nmol) & 50 OD (250 nmol)
• Purification: Desalt or HPLC
• Length: 19-27mer duplexes
• Modifications: Biotin, Phosphate, Amino-Modifier C7, 6-FAM, Cyanine 3, Cyanine 5, Cyanine 5.5, 2’-OMe, Phosphorothioate, Thiol-Modifier C6 S-S and Thiol-Modifier C3
• Quality Control: Individual strands are analyzed by mass spectrometry. Depending on the manufacturing site, PAGE may be used to assess miRNA duplexes. miRNA quantity is systematically validated by UV absorbance at 260 nm.
• For additional modifications and purification requests, email sirnarequest@sial.com

RNA Oligos

• Scale: 0.05, 0.2, 1.0 µmol
• Purification: Desalt, HPLC and PAGE
• Sequence Length: 7 - 45 bases
• Modification options available
• Quality Control: 100% Mass Spectrometry
• Format: Supplied dry
• Learn more about RNA Oligos

MISSION^® siRNA Transfection Reagent

• Superior gene silencing in a wide variety of eukaryotic cell types
• For use with siRNA, esiRNA, miRNA mimics and Synthetic miRNA inhibitors